A general strategy to develop cell membrane fluorescent probes with location- and target-specific fluorogenicities: a case of a Zn2+ probe with cellular selectivity

被引：30

作者：

Deng, Fei ^{[1
,2
]}

Liu, Limin ^{[1
]}

Qiao, Qinglong ^{[2
]}

Huang, Chunfang ^{[1
]}

Miao, Lu ^{[2
]}

Xu, Zhaochao ^{[2
]}

机构：

[1] Jinggangshan Univ, Sch Chem & Chem Engn, Jian 343009, Jiangxi, Peoples R China

[2] Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 100期

基金：

中国国家自然科学基金;

关键词：

ZINC INDICATOR; SURFACE; RELEASE; SENSOR;

D O I：

10.1039/c9cc08895d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We reported fluorescent probes to image Zn2+ with plasma membrane-specific and Zn2+-specific fluorogenicities. The probes contained hydrophobic alkyl chains as membrane-anchored domains and hydrophilic zinc sensor ZTRS, and aggregated to display quenched fluorescence. Cells dissolved the aggregates and the liberated probes were dispersed on the outside of the cell plasma membrane. Aggregates that did not bind to the cell membrane still exhibited aggregation-induced fluorescence quenching after complexing with zinc ions, while probes anchored on the membrane surface exhibited a fluorescence-enhanced response upon recognition of zinc ions.

引用

页码：15045 / 15048

页数：4

共 25 条

[1] Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging [J].

Chyan, Wen ;

Raines, Ronald T. .

ACS CHEMICAL BIOLOGY, 2018, 13 (07) :1810-1823

[2] Heteroatom-substituted rhodamine dyes: Structure and spectroscopic properties [J].

Deng, Fei ;

Xu, Zhaochao .

CHINESE CHEMICAL LETTERS, 2019, 30 (10) :1667-1681

[3] The environmental-sensitivity of a fluorescent <bold>ZTRS-Cd(ii)</bold> complex was applied to discriminate different types of surfactants and determine their CMC values [J].

Deng, Fei ;

Long, Shuangshuang ;

Qiao, Qinglong ;

Xu, Zhaochao .

CHEMICAL COMMUNICATIONS, 2018, 54 (48) :6157-6160

[4] Fluorescent probes for organelle-targeted bioactive species imaging [J].

Gao, Peng ;

Pan, Wei ;

Li, Na ;

Tang, Bo .

CHEMICAL SCIENCE, 2019, 10 (24) :6035-6071

[5] Development of a Cholesterol-Conjugated Fluorescent Sensor for Site-Specific Detection of Zinc Ion at the Plasma Membrane [J].

Iyoshi, Shohei ;

Taki, Masayasu ;

Yamamoto, Yukio .

ORGANIC LETTERS, 2011, 13 (17) :4558-4561

[6] Efficient cell surface labelling of live zebrafish embryos: wash-free fluorescence imaging for cellular dynamics tracking and nanotoxicity evaluation [J].

Jia, Hao-Ran ;

Zhu, Ya-Xuan ;

Xu, Ke-Fei ;

Pan, Guang-Yu ;

Liu, Xiaoyang ;

Qiao, Ying ;

Wu, Fu-Gen .

CHEMICAL SCIENCE, 2019, 10 (14) :4062-4068

[7] Toward a Chemical Marker for Inflammatory Disease: A Fluorescent Probe for Membrane-Localized Thioredoxin [J].

Lee, Min Hee ;

Jeon, Hyun Mi ;

Han, Ji Hye ;

Park, Nayoung ;

Kang, Chulhun ;

Sesser, Jonathan L. ;

Kim, Jong Seung .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (23) :8430-8437

[8] GLP-1 Receptor Mediated Targeting of a Fluorescent Zn2+ Sensor to Beta Cell Surface for Imaging Insulin/Zn2+ Release [J].

Li, Daliang ;

Huang, ZhiJiang ;

Chen, Shiuhwei ;

Hu, Zeping ;

Li, Wen-hong .

BIOCONJUGATE CHEMISTRY, 2015, 26 (08) :1443-1450

[9] Genetic Targeting of a Small Fluorescent Zinc Indicator to Cell Surface for Monitoring Zinc Secretion [J].

Li, Daliang ;

Liu, Lin ;

Li, Wen-Hong .

ACS Chemical Biology, 2015, 10 (04) :1054-1063

[10] Imaging dynamic insulin release using a fluorescent zinc indicator for monitoring induced exocytotic release (ZIMIR) [J].

Li, Daliang ;

Chen, Shiuhwei ;

Bellomo, Elisa A. ;

Tarasov, Andrei I. ;

Kaut, Callan ;

Rutter, Guy A. ;

Li, Wen-hong .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (52) :21063-21068

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