Effects of various spacers between biotin and the phospholipid headgroup on immobilization and sedimentation of biotinylated phospholipid-containing liposomes facilitated by avidin-biotin interactions

被引：4

作者：

Sakamoto, Yasuhisa ^{[1
]}

Kikuchi, Koji ^{[1
]}

Umeda, Kazuaki ^{[1
]}

Nakanishi, Hiroyuki ^{[1
]}

机构：

[1] Kumamoto Univ, Grad Sch Med Sci, Dept Mol Pharmacol, 1-1-1 Honjo, Kumamoto 8608556, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2017年 / 162卷 / 03期

基金：

日本学术振兴会;

关键词：

biotin; immobilization; liposome; sedimentation; spacer; STREPTAVIDIN MONOMER; PROTEIN; BINDING; PACKING;

D O I：

10.1093/jb/mvx016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilization and sedimentation of liposomes (lipid vesicles) are used in liposome-protein binding assays, facilitated by avidin/streptavidin/NeutrAvidin and biotinylated phospholipid-containing liposomes. Here, we examined the effects of three spacers [six-carbon (X), polyethylene glycol (PEG) 180 (molecular weight 180) and PEG2000 (molecular weight 2,000)] between biotin and the phospholipid headgroup on the immobilization and sedimentation of small unilamellar liposomes/vesicles (SUVs). PEG180 and PEG2000 showed more efficient immobilization of biotinylated SUVs on NeutrAvidin-coated plates than X, but X and PEG180 showed more efficient sedimentation of biotinylated SUVs upon NeutrAvidin addition than PEG2000. Thus, the most appropriate spacers differed between immobilization and sedimentation. A spacer for biotinylated SUVs must be selected according to the particular liposome-protein binding assays examined.

引用

页码：221 / 226

页数：6