Simultaneous fMRI and Local Field Potential Measurements During Epileptic Seizures in Medetomidine-Sedated Rats Using RASER Pulse Sequence

被引：37

作者：

Airaksinen, Antti M.

Niskanen, Juha-Pekka ^{[2
]}

Chamberlain, Ryan ^{[3
,4
]}

Huttunen, Joanna K.

Nissinen, Jan ^{[1
]}

Garwood, Michael ^{[3
,4
]}

Pitkanen, Asia ^{[1
,5
]}

Grohn, Olli ^{[1
]}

机构：

[1] Univ Eastern Finland, Dept Neurobiol, AI Virtanen Inst Mol Sci, Epilepsy Res Lab, FI-70211 Kuopio, Finland

[2] Univ Eastern Finland, Dept Math & Phys, FI-70211 Kuopio, Finland

[3] Univ Minnesota, Sch Med, Ctr Magnet Resonance Res, Minneapolis, MN 55455 USA

[4] Univ Minnesota, Sch Med, Dept Radiol, Minneapolis, MN 55455 USA

[5] Kuopio Univ Hosp, Dept Neurol, SF-70210 Kuopio, Finland

来源：

MAGNETIC RESONANCE IN MEDICINE | 2010年 / 64卷 / 04期

基金：

美国国家卫生研究院; 芬兰科学院;

关键词：

fMRI; RASER; medetomidine; local field potential; epilepsy; kainic acid; BOLD-FMRI; NEURONAL-ACTIVITY; ALPHA-CHLORALOSE; ABSENCE SEIZURES; ANESTHETIZED RAT; ANIMAL-MODEL; MRI; ANESTHESIA; BRAIN; CONNECTIVITY;

D O I：

10.1002/mrm.22508

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Simultaneous electrophysiological and functional magnetic resonance imaging measurements of animal models of epilepsy are methodologically challenging, but essential to better understand abnormal brain activity and hemodynamics during seizures. In this study, functional magnetic resonance imaging of medetomidine-sedated rats was performed using novel rapid acquisition by sequential excitation and refocusing (RASER) fast imaging pulse sequence and simultaneous local field potential measurements during kainic acid-induced seizures. The image distortion caused by the hippocampal-measuring electrode was clearly seen in echo planar imaging images, whereas no artifact was seen in RASER images. Robust blood oxygenation level-dependent responses were observed in the hippocampus during kainic acid-induced seizures. The recurrent epileptic seizures were detected in the local field potential signal after kainic acid injection. The presented combination of deep electrode local field potential measurements and functional magnetic resonance imaging under medetomidine anesthesia, which does not significantly suppress kainic acid-induced seizures, provides a unique tool for studying abnormal brain activity in rats. Magn Reson Med 64:1191-1199, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：1191 / 1199

页数：9

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