MOLECULAR CLONING, GENOMIC STRUCTURE, AND GENETIC MAPPING OF TWO RDL-ORTHOLOGOUS GENES OF GABA RECEPTORS IN THE DIAMONDBACK MOTH, Plutella xylostella

被引：31
作者：
Yuan, Guorui [1 ]
Gao, Weiyue [1 ]
Yang, Yihua [1 ]
Wu, Yidong [1 ]
机构：
[1] Nanjing Agr Univ, Coll Plant Protect, Minist Agr China, Key Lab Monitoring & Management Crop Dis & Pest I, Nanjing 210095, Peoples R China
来源：
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 2010年 / 74卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Plutella xylostella; Rdl; GABA receptor; alternative splicing; Z chromosome; ION-CHANNEL SUPERFAMILY; CROSS-RESISTANCE; FIPRONIL; LEPIDOPTERA; MUTATION; BIOLOGY; SEX;
D O I：
10.1002/arch.20361
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Resistance to dieldrin (Rdl) gene encodes a subunit of the insect gamma-aminobutyric acid (GABA) receptor. Cyclodiene resistance in many insects is associated with replacement of a single amino acid (alanine at position 302) with either a serine or a glycine in the Rdl gene. Two Rdl-orthologous genes of GABA receptors (PxGABAR alpha 1 and PxGABAR alpha 2) were cloned and sequenced from a susceptible strain (Roth) of Plutella xylostella. PxGABAR alpha 1 and PxGABAR alpha 2 showed 84% and 77% identity with the Rdl gene of Drosophila melanogaster at an amino acid level, respectively. The coding regions of PxGABAR alpha 1 and PxGA-BAR alpha 2 both comprise ten exons, with two alternative RNA-splicing forms in exon 3 of both genes. At the orthologous position of alanine-302 in D. melanogaster Rdl, PxGABAR alpha 1 has a conserved alanine at position 282. PxGABAR alpha 2 has a serine instead of an alanine at the equivalent
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页码：81 / 90
页数：10
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