Parameters guiding the self-assembly of quantum dots and DNA origami by peptide-PNA

被引：0

作者：

Green, Christopher M. ^{[1
]}

Hastman, David A. ^{[1
]}

Mathur, Divita ^{[1
]}

Susumu, Kimihiro ^{[2
]}

Medintz, Igor L. ^{[1
]}

Diaz, Sebastian A. ^{[1
]}

机构：

[1] US Naval Res Lab, Ctr Bio Mol Sci & Engn, Code 6900, Washington, DC 20375 USA

[2] US Naval Res Lab, Opt Sci Div, Code 5600, Washington, DC 20375 USA

来源：

2021 IEEE 21ST INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY (IEEE NANO 2021) | 2021年

关键词：

D O I：

10.1109/NANO51122.2021.9514331

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Nanoparticle (NP)-binding peptides conjugated to peptide nucleic acids - peptide-PNA - enable efficient and programmable self-assembly of quantum dots (QDs) on DNA nanostructures. As an alternative to chemically modified DNA, peptide-PNA were designed with a poly-histidine peptide motif (Histag) to enable self-assembly to the surface of ZnS-shelled QDs, along with a PNA domain which could undergo hybridization to a complementary DNA sequence by Watson-Crick base pairing. We have demonstrated that QDs can be conjugated efficiently to DNA origami by peptide-PNA without requiring a large excess of the individual components. Here, optimization was performed to assess the effects of varied molar ratios of peptide-PNA:QDs and QDs:origami on the binding efficiency of QDs to DNA origami.

引用

页码：448 / 450

页数：3

共 14 条

[1] Programmable Periodicity of Quantum Dot Arrays with DNA Origami Nanotubes [J].

Bui, Hieu ;

Onodera, Craig ;

Kidwell, Carson ;

Tan, YerPeng ;

Graugnard, Elton ;

Kuang, Wan ;

Lee, Jeunghoon ;

Knowlton, William B. ;

Yurke, Bernard ;

Hughes, William L. .

NANO LETTERS, 2010, 10 (09) :3367-3372

[2] Elucidating Surface Ligand-Dependent Kinetic Enhancement of Proteolytic Activity at Surface-Modified Quantum Dots [J].

Diaz, Sebastian A. ;

Sen, Soumyo ;

Gemmill, Kelly Boeneman ;

Brown, Carl W., III ;

Oh, Eunkeu ;

Susumu, Kimihiro ;

Stewart, Michael H. ;

Breger, Joyce C. ;

Aragones, Guillermo Lasarte ;

Field, Lauren D. ;

Deschamps, Jeffrey R. ;

Kral, Petr ;

Medintz, Igor L. .

ACS NANO, 2017, 11 (06) :5884-5896

[3] Direct and Efficient Conjugation of Quantum Dots to DNA Nanostructures with Peptide-PNA [J].

Green, Christopher M. ;

Hastman, David A. ;

Mathur, Divita ;

Susumu, Kimihiro ;

Oh, Eunkeu ;

Medintz, Igor L. ;

Diaz, Sebastian A. .

ACS NANO, 2021, 15 (05) :9101-9110

[4] Peptide nucleic acids (PNA): Synthesis, properties and potential applications [J].

Hyrup, B ;

Nielsen, PE .

BIOORGANIC & MEDICINAL CHEMISTRY, 1996, 4 (01) :5-23

[5] Nanomanufacturing with DNA Origami: Factors Affecting the Kinetics and Yield of Quantum Dot Binding [J].

Ko, Seung Hyeon ;

Gallatin, Gregg M. ;

Liddle, J. Alexander .

ADVANCED FUNCTIONAL MATERIALS, 2012, 22 (05) :1015-1023

[6] DNA Origami Route for Nanophotonics [J].

Kuzyk, Anton ;

Jungmann, Ralf ;

Acuna, Guillermo P. ;

Liu, Na .

ACS PHOTONICS, 2018, 5 (04) :1151-1163

[7] Chemistries for DNA Nanotechnology [J].

Madsen, Mikael ;

Gothelf, Kurt V. .

CHEMICAL REVIEWS, 2019, 119 (10) :6384-6458

[8] Self-assembled nanoscale biosensors based on quantum dot FRET donors [J].

Medintz, IL ;

Clapp, AR ;

Mattoussi, H ;

Goldman, ER ;

Fisher, B ;

Mauro, JM .

NATURE MATERIALS, 2003, 2 (09) :630-638

[9] Quantum dot bioconjugates for imaging, labelling and sensing [J].

Medintz, IL ;

Uyeda, HT ;

Goldman, ER ;

Mattoussi, H .

NATURE MATERIALS, 2005, 4 (06) :435-446

[10] PEPTIDE NUCLEIC-ACID (PNA) - A DNA MIMIC WITH A PEPTIDE BACKBONE [J].

NIELSEN, PE ;

EGHOLM, M ;

BUCHARDT, O .

BIOCONJUGATE CHEMISTRY, 1994, 5 (01) :3-7

← 1 2 →