Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil

被引：0

作者：

Walker, MJ ^{[1
]}

Montemagno, C

Bryant, JC

Ghiorse, WC

机构：

[1] Univ Nevada, Dept Environm & Resource Sci, Reno, NV 89557 USA

[2] Cornell Univ, Dept Agr & Biol Engn, Ithaca, NY 14853 USA

[3] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA

[4] Cornell Univ, Microbiol Sect, Div Biol Sci, Ithaca, NY 14853 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1998年 / 64卷 / 06期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We determined and compared the method detection limits (MDLalpha) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDLalpha and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil studies of oocyst transport.

引用

页码：2281 / 2283

页数：3

共 12 条

[1] Computer-assisted laser scanning and video microscopy for analysis of Cryptosporidium parvum oocysts in soil, sediment, and feces [J].

Anguish, LJ ;

Ghiorse, WC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (02) :724-733

[2]

BROWN PJ, 1993, OXFORD STAT SERIES, V12

[3]

BRUSH CF, 1997, THESIS CORNELL U ITH

[4]

Clesceri L.S., 1989, STANDARD METHODS EXA, V17th

[5] A NOTE ON CONFIDENCE BANDS FOR THE LOGISTIC RESPONSE CURVE [J].

HAUCK, WW .

AMERICAN STATISTICIAN, 1983, 37 (02) :158-160

[6]

Innis MA., 1990, PCR PROTOCOLS, P3

[7] Assessment of a dye permeability assay for determination of inactivation rates of Cryptosporidium parvum oocysts [J].

Jenkins, MB ;

Anguish, LJ ;

Bowman, DD ;

Walker, MJ ;

Ghiorse, WC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (10) :3844-3850

[8] DEVELOPMENT OF A PCR PROTOCOL FOR SENSITIVE DETECTION OF CRYPTOSPORIDIUM OOCYSTS IN WATER SAMPLES [J].

JOHNSON, DW ;

PIENIAZEK, NJ ;

GRIFFIN, DW ;

MISENER, L ;

ROSE, JB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (11) :3849-3855

[9] Movement of the protozoan pathogen Cryptosporidium parvum through three contrasting soil types [J].

Mawdsley, JL ;

Brooks, AE ;

Merry, RJ .

BIOLOGY AND FERTILITY OF SOILS, 1996, 21 (1-2) :30-36

[10] Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater [J].

Mayer, CL ;

Palmer, CJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (06) :2081-2085

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