Morphological and neuro-behavioral parallels in the rat model of stroke

被引：34

作者：

Boyko, Matthew ^{[1
]}

Ohayon, Sharon ^{[1
]}

Goldsmith, Tomer ^{[1
]}

Novack, Lena ^{[2
]}

Novack, Victor ^{[3
]}

Perry, Zvi H. ^{[4
]}

Gruenbaum, Benjamin F. ^{[1
]}

Gruenbaum, Shaun E. ^{[5
]}

Steiner, Oded ^{[1
]}

Shapira, Yoram ^{[1
]}

Teichberg, Vivian I. ^{[6
]}

Zlotnik, Alexander ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Soroka Med Ctr, Div Anesthesiol & Crit Care, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Fac Hlth Sci, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Soroka Med Ctr, Clin Res Ctr, IL-84105 Beer Sheva, Israel

[4] Ben Gurion Univ Negev, Epidemiol & Hlth Evaluat Dept, Surg Dept A, IL-84105 Beer Sheva, Israel

[5] Yale Univ, Sch Med, Dept Anesthesiol, New Haven, CT 06510 USA

[6] Weizmann Inst Sci, Dept Neurobiol, IL-76100 Rehovot, Israel

来源：

BEHAVIOURAL BRAIN RESEARCH | 2011年 / 223卷 / 01期

关键词：

Brain injury; Middle cerebral artery occlusion; Neurological severity score; Rats; Stroke; MIDDLE CEREBRAL-ARTERY; NEUROLOGICAL DEFICIT; INTRALUMINAL SUTURE; INFARCT SIZE; OCCLUSION; ISCHEMIA; IMPAIRMENT; APOPTOSIS; INJURY; VOLUME;

D O I：

10.1016/j.bbr.2011.03.019

中图分类号：

B84 [心理学]; C [社会科学总论]; Q98 [人类学];

学科分类号：

03 ; 0303 ; 030303 ; 04 ; 0402 ;

摘要：

Middle cerebral artery occlusion (MCAO) is widely used as a rat model of focal brain ischemia. Evaluation of brain damage often includes the morphological analysis of the injury area, MRI, and various scales which depend on functional tests, commonly known as neurological severity score (NSS). We determined the optimal number of NSS tests and assessed their capacity for non-invasive evaluation of brain ischemic injury in the rat MCAO model. 275 male Sprague-Dawley rats were randomly divided into five groups, given either permanent (p) MCAO or transient (t) MCAO using an uncoated 4-0 monofilament catheter or a silicone-coated monofilament. The rats neurological status was examined before and at 1 and 24 h following MCAO. The size of brain injury was then measured histologically and the extent of right cerebral hemisphere edema was calculated. We established a correlation between these tests and morphological data for brain injury. Adjusted R-2 of the prediction of total histology score was 0.7. The Hosmer-Lemeshow p-value of this model was 0.812 for total brain histology. For the brain edema the adjusted R-2 of the prediction model was 0.48. The Hosmer-Lemeshow p-value of this model was 0.558 for brain edema. Our methods of estimating infarct size produces reliable and well correlated results at 24 h and demonstrates to be an easy and quick way to assess infarct size soon after ischemic injury has occurred. The described method for neurological assessment could ultimately aid in assessing various treatment modalities in the early hours following stroke. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：17 / 23

页数：7

共 31 条

[1] Multi-modal characterisation of the neocortical clip model of focal cerebral ischaemia by MRI, behaviour and immunohistochemistry [J].