Pseudomonas fluorescens BBc6R8 type III secretion mutants no longer promote ectomycorrhizal symbiosis

被引：41
作者：
Cusano, Angela M. [1 ]
Burlinson, Peter [1 ,2 ]
Deveau, Aurelie [3 ]
Vion, Patrice [1 ]
Uroz, Stephane [1 ]
Preston, Gail M. [2 ]
Frey-Klett, Pascale [1 ]
机构：
[1] Nancy Univ, INRA, UMR1136, Ctr Nancy,IFR110, F-54280 Champenoux, France
[2] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
[3] Dartmouth Med Sch, Dept Microbiol & Immunol, Hanover, NH 03755 USA
来源：
ENVIRONMENTAL MICROBIOLOGY REPORTS | 2011年 / 3卷 / 02期
关键词：
MYCORRHIZA HELPER BACTERIA; FUNGUS LACCARIA-BICOLOR; GENE-EXPRESSION; SYSTEM; GROWTH; SURVIVAL; EFFECTORS; PROTEINS; PLANTS; MHB;
D O I：
10.1111/j.1758-2229.2010.00209.x
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
P>The Mycorrhiza Helper Bacterium (MHB) Pseudomonas fluorescens BBc6R8 promotes the ectomycorrhizal symbiosis between Douglas fir roots and Laccaria bicolor. In this study, we identified a non-flagellar type III secretion system (T3SS) in the draft genome of BBc6R8 similar to that described in the biocontrol strain P. fluorescens SBW25. We examined whether this T3SS plays a role in the BBc6R8 mycorrhizal helper effect by creating a deletion in the rscRST genes encoding the central channel of the injectisome. The in vitro effect of BBc6R8 T3SS mutants on the radial growth rate of L. bicolor was unchanged compared with the parental strain. In contrast, T3SS mutants were unable to promote mycorrhization, suggesting that type III secretion plays an important role in the mycorrhizal helper effect of P. fluorescens BBc6R8 independent of the promotion of hyphal growth that BBc6R8 exhibits in vitro.
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收藏
页码：203 / 210
页数：8
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