Mapping novel QTLs for yield related traits from a popular rice hybrid KRH-2 derived doubled haploid (DH) population

被引：4

作者：

Kulkarni, Swapnil Ravindra ^{[1
]}

Balachandran, S. M. ^{[1
]}

Ulaganathan, K. ^{[2
]}

Balakrishnan, Divya ^{[1
]}

Prasad, A. S. Hari ^{[1
]}

Rekha, G. ^{[1
]}

Kousik, M. B. V. N. ^{[1
]}

Hajira, S. K. ^{[1
]}

Kale, Ravindra Ramarao ^{[1
]}

Aleena, D. ^{[1
]}

Anila, M. ^{[1
]}

Punniakoti, E. ^{[1
]}

Dilip, T. ^{[1
]}

Pranathi, K. ^{[1
]}

Das, M. Ayyappa ^{[1
]}

Shaik, Mastanbee ^{[1
]}

Chaitra, K. ^{[1
]}

Sinha, Pragya ^{[1
]}

Sundaram, R. M. ^{[1
]}

机构：

[1] ICAR Indian Inst Rice Res, Biotechnol Dept, Hyderabad 500030, Telangana, India

[2] Osmania Univ, Ctr Plant Mol Biol CPMB, Hyderabad 500007, Andhra Pradesh, India

来源：

3 BIOTECH | 2021年 / 11卷 / 12期

关键词：

Doubled haploid lines (DHLs); SSR markers; Inclusive composite interval mapping (ICIM); Chromosomal regions with gene clusters; ADVANCED BACKCROSS POPULATION; GRAIN-YIELD; AGRONOMIC TRAITS; ORYZA-RUFIPOGON; DROUGHT STRESS; PROTEIN GENE; DOF PROTEIN; LOCI; IDENTIFICATION; COMPONENTS;

D O I：

10.1007/s13205-021-03045-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A doubled haploid (DH) population consisting of 125 DHLs derived from the popular rice hybrid, KRH-2 (IR58025A/KMR3R) was utilized for Quantitative Trait Loci (QTL) mapping to identify novel genomic regions associated with yield related traits. A genetic map was constructed with 126 polymorphic SSR and EST derived markers, which were distributed across rice genome. QTL analysis using inclusive composite interval mapping (ICIM) method identified a total of 24 major and minor effect QTLs. Among them, twelve major effect QTLs were identified for days to fifty percent flowering (qDFF12-1), total grain yield/plant (qYLD3-1 and qYLD6-1), test (1,000) grain weight (qTGW6-1 and qTGW7-1), panicle weight (qPW9-1), plant height (qPH12-1), flag leaf length (qFLL6-1), flag leaf width (qFLW4-1), panicle length (qPL3-1 and qPL6-1) and biomass (qBM4-1), explaining 29.95-56.75% of the phenotypic variability with LOD scores range of 2.72-16.51. Chromosomal regions with gene clusters were identified on chromosome 3 for total grain yield/plant (qYLD3-1) and panicle length (qPL3-1) and on chromosome 6 for total grain yield/plant (qYLD6-1), flag leaf length (qFLL6-1) and panicle length (qPL6-1). Majority of the QTLs identified were observed to be co-localized with the previously reported QTL regions. Five novel, major effect QTLs associated with panicle weight (qPW9-1), plant height (qPH12-1), flag leaf width (qFLW4-1), panicle length (qPL3-1) and biomass (qBM4-1) and three novel minor effect QTLs for panicle weight (qPW3-1 and qPW8-1) and fertile grains per panicle (qFGP5-1) were identified. These QTLs can be used in breeding programs aimed to yield improvement after their validation in alternative populations.

引用

页数：20

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