Detecting penicillin G in milk with impedimetric label-free immunosensor

被引：79

作者：

Thavarungkul, Panote ^{[1
,2
]}

Dawan, Supapom ^{[2
,3
]}

Kanatharana, Proespichaya ^{[2
,3
]}

Asawatreratanakul, Punnee ^{[2
,4
]}

机构：

[1] Prince Songkla Univ, Fac Sci, Dept Phys, Hat Yai 90112, Songkhla, Thailand

[2] Prince Songkla Univ, Biophys Res Unit Biosensors & Biocurrents, Hat Yai 90112, Songkhla, Thailand

[3] Prince Songkla Univ, Fac Sci, Dept Chem, Hat Yai 90112, Songkhla, Thailand

[4] Prince Songkla Univ, Fac Sci, Dept Biochem, Hat Yai 90112, Songkhla, Thailand

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 23卷 / 05期

关键词：

impedimetric; immunosensor; penicillin G; benzylpenicillin; label-free; flow injection;

D O I：

10.1016/j.bios.2007.08.003

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A label-free impedimetric flow injection immunosensor for the direct detection of penicillin G has been developed. Anti-penicillin G was immobilized on a gold working electrode modified with a self-assembled monolayer of thioctic acid. Real time monitoring of impedance was carried out at the optimum frequency of 160 Hz. Under optimum operating conditions the system provided a wide linear range between 1.0 x 10(-13) and 1.0 x 10(-8) M with a very low detection limit of 3.0 x 10(-15) M, much lower than the MRL of penicillin G in milk (1.2 x 10(-8) M). The immobilized anti-penicillin G on self-assembled thioctic acid monolayer gold electrode was very stable and provided good reproducible signal after regeneration up to 45 times with a relative standard deviation (R.S.D.) lower than 4%. Good recoveries and precisions were obtained when spiked raw milk samples were analyzed. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：688 / 694

页数：7

共 26 条

[1] On the response of a label-free interferon-γ immunosensor utilizing electrochemical impedance spectroscopy [J].