Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis

被引：23

作者：

Kozlowski, David J. ^{[1
,2
]}

Chen, Zhong ^{[3
]}

Zhuang, Lina ^{[3
]}

Fei, You-Jun ^{[3
]}

Navarre, Sammy ^{[1
,2
]}

Ganapathy, Vadivel ^{[3
]}

机构：

[1] Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA

[2] Med Coll Georgia, Inst Mol Med & Genet, Augusta, GA 30912 USA

[3] Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA

来源：

LIFE SCIENCES | 2008年 / 82卷 / 19-20期

关键词：

taurine transporter; zebrafish; embryogenesis; retina; brain; apoptosis;

D O I：

10.1016/j.lfs.2008.02.015

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na+/Cl--dependent manner with a Na+:Cl-:taurine stoichiometry of 2:1:1. In the zebrafish early cleavage stages, indicating that both the embryo, taurine and TauT mRNA are present during. transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：1004 / 1011

页数：8

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