Alum-type Adjuvant Effect of Non-haemolytic Saponins Purified from Ilex and Passiflora spp.

被引:8
作者
Silveira, F. [1 ]
Rossi, S. [2 ]
Fernandez, C. [2 ]
Gosmann, G. [3 ]
Schenkel, E. [4 ]
Ferreira, F. [1 ]
机构
[1] Fac Med Quim, Dept Quim Organ, Lab Carbohidratos & Glicoconjugados, Dept Desarrollo Biotecnol,Inst Higiene, Montevideo 11600, Uruguay
[2] Fac Quim Montevideo, Inst Higiene, Catedra Inmunol, Montevideo 11600, Uruguay
[3] Univ Fed Rio Grande do Sul, Fac Farm, BR-90610000 Porto Alegre, RS, Brazil
[4] Univ Fed Santa Catarina, Ctr Ciencias Saude, Dept Ciencias Farmaceut, BR-88040970 Florianopolis, SC, Brazil
关键词
Ilex; Passiflora; saponins; adjuvant; haemolysis; tetanus toxoid; VACCINE ADJUVANTS; ALATA; PARAGUARIENSIS; MATE; TEA;
D O I
10.1002/ptr.3454
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Five saponins purified from the leaves of three Ilex species (saponins 1 and 2 from I. dumosa; saponin 3 from I. argentina; saponin 4 from I. paraguariensis) and from Passiflora alata (saponin 5) were evaluated for their in vitro haemolytic activity and in vivo immunostimulatory ability in a mouse model using tetanus toxoid (TT) as a model antigen. The assayed saponins showed very weak or no haemolytic activity over the tested concentration range. Mice were immunized twice with TT formulated with pure saponins 1-5, or with a mixture of saponins from Quillaja saponaria, aluminum hydroxide gel or saline, which were used as controls. The elicited humoral response was evaluated by means of the time course of specific serum antibody levels up to day 131 post-priming (total IgG and isotypes); the cellular response was tested through a delayed-type hypersensitivity (DTH) assay. The assayed saponins, in particular saponins 3 and 5, showed an adjuvant effect similar to that of alum for all tested parameters. The immunostimulating potential of these compounds deserves further investigation, especially taking into account that some Ilex spp. and Passiflora alata are native crops of widespread use and economical importance in Latin America. Copyright (C) 2011 John Wiley & Sons, Ltd.
引用
收藏
页码:1783 / 1788
页数:6
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