Affinity ionic liquids for the rapid liquid-liquid extraction purification of hexahistidine tagged proteins

被引：21
作者：
Ren, Guangwei [1 ]
Gong, Xingwen [1 ]
Wang, Bilei [1 ]
Chen, Yingyi [1 ]
Huang, Jianying [1 ]
机构：
[1] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Fruits & Vegetables Postharvest & Proc Te, Hangzhou 310018, Zhejiang, Peoples R China
来源：
SEPARATION AND PURIFICATION TECHNOLOGY | 2015年 / 146卷
基金：
美国国家科学基金会;
关键词：
Affinity ionic liquids; Liquid-liquid extraction; Triazacyclononane; His-tagged proteins; Purification; AQUEOUS 2-PHASE SYSTEMS; BINDING; CHROMATOGRAPHY; POLYMER; FLUORESCENCE; LYSOZYME; CAPACITY; STRENGTH; BEHAVIOR; PEPTIDE;
D O I：
10.1016/j.seppur.2015.03.025
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
A triazacyclononane-ionic liquid (IL)-based sorbent was synthesized, characterized, and applied for rapid and inexpensive purification of hexahistidine tagged (His-tagged) proteins via liquid-liquid extraction. Selective partition of the target proteins between the IL phase and aqueous phase highly depends on a variety of parameters, such as the affinity ionic liquid (AIL), coordinated metal ions, and ionic strength. Compared with the chromatographic properties of Cu2+-AIL/IL and Ni2+-AIL/IL, Zn2+-AIL/IL exhibited relatively higher binding capacity to separate the proteins with high purity (up to 95%). A reclamation method for the AIL/IL extraction sorbent was further established by washing with ethylene diamine tetraacetic acid (EDTA) and then re-immobilization of metal ions. The purification strategies described herein were mild and suitable for a large scale separation of biologically active macromolecules with the possibility of continuous processing and a reduction in operational cost. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：114 / 120
页数：7
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