Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics

被引：8

作者：

Li, Qiyan ^{[1
]}

Zhu, Riran ^{[2
]}

Li, Jun ^{[1
]}

Wang, Xiaobing ^{[1
]}

Xu, Lihua ^{[1
]}

Li, Yanshen ^{[3
]}

Li, Peng ^{[4
]}

机构：

[1] Shandong Inst Food & Drug Control, Hlth Food & Cosmet Lab, Jinan 250101, Peoples R China

[2] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Fac Pharmaceut Sci, Jinan 250012, Peoples R China

[3] Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China

[4] Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Milk & Dairy Prod, Beijing 100193, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY | 2019年 / 2019卷

基金：

欧盟地平线“2020”; 中国国家自然科学基金;

关键词：

FLORFENICOL AMINE; LC-MS/MS; RESIDUES; METRONIDAZOLE; THIAMPHENICOL; TOXICITY; SAMPLES; ELISA;

D O I：

10.1155/2019/7131907

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed. The anti-chloramphenicol antibody (mAb) adopted in thiswork for direct immunoassay could bind to CAP specifically, with negligible cross-reactivity (CR) (less than 0.01%) with most CAP analogues, including structurally related thiamphenicol (TAP) and florfenicol (FF). The limit of detection (LOD), measured by IC10, was 0.0021 ng mL(-1). The detection range (IC20-IC80) was ranged from 0.00979 to 0.12026 ng mL(-1). In spiked cosmetics samples, mean recoveries ranged from 82.7% to 99.6%, with intraday and interday variation less than 9.8 and 8.2%, respectively. Moreover, with the help of HRP-labeled anti-CAPmAb, the method could be processed in fast direct immunoreaction mode. This CL-ELISA method could be applied for specific, rapid, semiquantitative, and quantitative detection of CAP in cosmetics, facilitating the precise quality control of CAP contamination.

引用

页数：6

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