Metagenome - Inferred bacterial replication rates in cystic fibrosis airways

被引：5

作者：

Pienkowska, Katarzyna ^{[1
]}

Wiehlmann, Lutz ^{[1
,2
]}

Tuemmler, Burkhard ^{[1
,3
]}

机构：

[1] Hannover Med Sch, Clin Pediat Pneumol Allergol & Neonatol, Carl Neuberg Str 1, D-30625 Hannover, Germany

[2] Hannover Med Sch, Reseach Core Unit Genom, D-30625 Hannover, Germany

[3] German Ctr Lung Res, Biomed Res Endstage & Obstruct Lung Dis BREATH, D-30625 Hannover, Germany

来源：

JOURNAL OF CYSTIC FIBROSIS | 2019年 / 18卷 / 05期

关键词：

Bacterial growth; Airway metagenome; Microbiome; Replication; Pseudomonas aeruginosa; Staphylococats aureus; Streptococci; Cystic fibrosis; MICROBIOME; GUT;

D O I：

10.1016/j.jcf.2019.01.003

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Bacterial replication rates were determined from metagenome sequencing of nasal lavage, throat swabs and induced sputa collected from healthy subjects and individuals with COPD or cystic fibrosis. More than 90% of peakto-trough coverage ratios of major clones were above 1.4 indicating that the most abundant bacterial species in the microbial communities were replicating in the airways including common inhabitants such as Prevotella and Streptococcus species as well as the cystic fibrosis pathogens Staphylococcus aureus and Pseudomonas aeruginosa. The populations of P. aeruginosa and S. aureus were replicating their pool of chromosomes more slowly than the populations of the common inhabitants of a healthy airway microbial flora. The assessment of growth dynamics in microbial metagenomes could become a decision-making tool for the diagnosis and management of bacterial infections in cystic fibrosis. (C) 2019 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

引用

页码：653 / 656

页数：4

共 28 条

[1] A Different Microbiome Gene Repertoire in the Airways of Cystic Fibrosis Patients with Severe Lung Disease [J].