A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is Insensitive to Antiretroviral Drugs

被引：4

作者：

Huang, Deli ^{[1
]}

Tran, Jenny Tuyet ^{[1
]}

Peng, Linghang ^{[1
]}

Yang, Linlin ^{[1
]}

Suhandynata, Raymond T. ^{[2
]}

Hoffman, Melissa A. ^{[2
]}

Zhao, Fangzhu ^{[1
,3
,4
]}

Song, Ge ^{[1
,3
,4
]}

He, Wan-Ting ^{[1
,3
,4
]}

Limbo, Oliver ^{[3
,5
]}

Callaghan, Sean ^{[1
,3
,4
]}

Landais, Elise ^{[1
,3
,4
,5
]}

Andrabi, Raiees ^{[1
,3
,4
]}

Sok, Devin ^{[1
,3
,4
,5
]}

Jardine, Joseph G. ^{[3
,5
]}

Burton, Dennis R. ^{[1
,3
,4
,6
]}

Voss, James E. ^{[1
]}

Fitzgerald, Robert L. ^{[2
]}

Nemazee, David ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Immunol & Microbiol, La Jolla, CA 92037 USA

[2] Univ Calif San Diego Hlth, Dept Pathol, San Diego, CA USA

[3] Scripps Res Inst, Int Aids Vaccine Initiat Neutralizing Antibody Ct, La Jolla, CA 92037 USA

[4] Scripps Res Inst, Consortium HIV AIDS Vaccine Dev, La Jolla, CA 92037 USA

[5] Int AIDS Vaccine Initiat, New York, NY USA

[6] Ragon Inst MGH MIT & Harvard, Cambridge, MA USA

来源：

JOURNAL OF IMMUNOLOGY | 2021年 / 207卷 / 01期

基金：

美国国家卫生研究院;

关键词：

INFECTION; SPIKE;

D O I：

10.4049/jimmunol.2100155

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike pseudotyped virus (PSV) assays are widely used to measure neutralization titers of sera and of isolated neutralizing Abs (nAbs). PSV neutralization assays are safer than live virus neutralization assays and do not require access to biosafety level 3 laboratories. However, many PSV assays are nevertheless somewhat challenging and require at least 2 d to carry out. In this study, we report a rapid (<30 min), sensitive, cell-free, off-the-shelf, and accurate assay for receptor binding domain nAb detection. Our proximity-based luciferase assay takes advantage of the fact that the most potent SARS-CoV-2 nAbs function by blocking the binding between SARS-CoV-2 and angiotensin-converting enzyme 2. The method was validated using isolated nAbs and sera from spike-immunized animals and patients with coronavirus disease 2019. The method was particularly useful in patients with HIV taking antiretroviral therapies that interfere with the conventional PSV assay. The method provides a cost-effective and point-of-care alternative to evaluate the potency and breadth of the predominant SARS-CoV-2 nAbs elicited by infection or vaccines.

引用

页码：344 / 351

页数：9

共 50 条

[21] Neutralizing antibodies against SARS-CoV-2 of vaccinated healthcare workers in Taiwan [J].

Priyambodo, Seto ;

Kuo, Kuang-Che ;

Weng, Ken-Pen ;

Liu, Shih-Feng ;

Syu, Guan-Da ;

Kuo, Ho-Chang .

ANNALS OF MEDICINE, 2025, 57 (01)

[22] Comprehensive Overview of Broadly Neutralizing Antibodies against SARS-CoV-2 Variants [J].

Cui, Lingyan ;

Li, Tingting ;

Xue, Wenhui ;

Zhang, Sibo ;

Wang, Hong ;

Liu, Hongjing ;

Gu, Ying ;

Xia, Ningshao ;

Li, Shaowei .

VIRUSES-BASEL, 2024, 16 (06)

[23] Neutralizing antibodies to SARS-CoV-2 variants of concern: a pediatric surveillance study [J].

Fraser, Douglas D. ;

Singh, Devika ;

Cela, Enis ;

Patel, Maitray A. ;

Assaf, Maysaa ;

Quintero, Maritza ;

Knauer, Michael ;

Miller, Michael R. ;

Bellini, Melina ;

Li, Angela ;

Hahn, Paul ;

Hrek, Marta ;

Cruz-Aguado, Jorge .

SCIENTIFIC REPORTS, 2025, 15 (01)

[24] Evaluation of Binding and Neutralizing Antibodies for Inactivated SARS-CoV-2 Vaccine Immunization [J].

Zhao, Heng ;

Jiang, Guorun ;

Li, Cong ;

Che, Yanchun ;

Long, Runxiang ;

Pu, Jing ;

Zhang, Ying ;

Li, Dandan ;

Liao, Yun ;

Yu, Li ;

Zhao, Yong ;

Yuan, Mei ;

Li, Yadong ;

Fan, Shengtao ;

Liu, Longding ;

Li, Qihan .

DISEASES, 2024, 12 (04)

[25] Signatures in SARS-CoV-2 spike protein conferring escape to neutralizing antibodies [J].

Alenquer, Marta ;

Ferreira, Filipe ;

Lousa, Diana ;

Valerio, Mariana ;

Medina-Lopes, Monica ;

Bergman, Marie-Louise ;

Goncalves, Juliana ;

Demengeot, Jocelyne ;

Leite, Ricardo B. ;

Lilue, Jingtao ;

Ning, Zemin ;

Penha-Goncalves, Carlos ;

Soares, Helena ;

Soares, Claudio M. ;

Amorim, Maria Joao .

PLOS PATHOGENS, 2021, 17 (08)

[26] Importance, Applications and Features of Assays Measuring SARS-CoV-2 Neutralizing Antibodies [J].

Gattinger, Pia ;

Ohradanova-Repic, Anna ;

Valenta, Rudolf .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (06)

[27] Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift [J].

Cameroni, Elisabetta ;

Bowen, John E. ;

Rosen, Laura E. ;

Saliba, Christian ;

Zepeda, Samantha K. ;

Culap, Katja ;

Pinto, Dora ;

VanBlargan, Laura A. ;

De Marco, Anna ;

di Iulio, Julia ;

Zatta, Fabrizia ;

Kaiser, Hannah ;

Noack, Julia ;

Farhat, Nisar ;

Czudnochowski, Nadine ;

Havenar-Daughton, Colin ;

Sprouse, Kaitlin R. ;

Dillen, Josh R. ;

Powell, Abigail E. ;

Chen, Alex ;

Maher, Cyrus ;

Yin, Li ;

Sun, David ;

Soriaga, Leah ;

Bassi, Jessica ;

Silacci-Fregni, Chiara ;

Gustafsson, Claes ;

Franko, Nicholas M. ;

Logue, Jenni ;

Iqbal, Najeeha Talat ;

Mazzitelli, Ignacio ;

Geffner, Jorge ;

Grifantini, Renata ;

Chu, Helen ;

Gori, Andrea ;

Riva, Agostino ;

Giannini, Olivier ;

Ceschi, Alessandro ;

Ferrari, Paolo ;

Cippa, Pietro E. ;

Franzetti-Pellanda, Alessandra ;

Garzoni, Christian ;

Halfmann, Peter J. ;

Kawaoka, Yoshihiro ;

Hebner, Christy ;

Purcell, Lisa A. ;

Piccoli, Luca ;

Pizzuto, Matteo Samuele ;

Walls, Alexandra C. ;

Diamond, Michael S. .

NATURE, 2022, 602 (7898) :664-+

[28] Development and characterization of SARS-CoV-2 variant-neutralizing monoclonal antibodies [J].

Qiu, Hongyu ;

Yuan, Xin-Yong ;

Cabral, Teresa ;

Manguiat, Kathy ;

Robinson, Alyssia ;

Wood, Heidi ;

Grant, Chris ;

McQueen, Peter ;

Westmacott, Garrett ;

Beniac, Daniel R. ;

Lin, Lisa ;

Carpenter, Michael ;

Kobasa, Darwyn ;

Grafenhan, Tom .

ANTIVIRAL RESEARCH, 2021, 196

[29] SARS-COV-2 recombinant Receptor-Binding-Domain (RBD) induces neutralizing antibodies against variant strains of SARS-CoV-2 and SARS-CoV-1 [J].

Law, John Lok Man ;

Logan, Michael ;

Joyce, Michael A. ;

Landi, Abdolamir ;

Hockman, Darren ;

Crawford, Kevin ;

Johnson, Janelle ;

LaChance, Gerald ;

Saffran, Holly A. ;

Shields, Justin ;

Hobart, Eve ;

Brassard, Raelynn ;

Arutyunova, Elena ;

Pabbaraju, Kanti ;

Croxen, Matthew ;

Tipples, Graham ;

Lemieux, M. Joanne ;

Tyrrell, D. Lorne ;

Houghton, Michael .

VACCINE, 2021, 39 (40) :5769-5779

[30] Cross-neutralizing antibodies bind a SARS-CoV-2 cryptic site and resist circulating variants [J].

Li, Tingting ;

Xue, Wenhui ;

Zheng, Qingbing ;

Song, Shuo ;

Yang, Chuanlai ;

Xiong, Hualong ;

Zhang, Sibo ;

Hong, Minqing ;

Zhang, Yali ;

Yu, Hai ;

Zhang, Yuyun ;

Sun, Hui ;

Huang, Yang ;

Deng, Tingting ;

Chi, Xin ;

Li, Jinjin ;

Wang, Shaojuan ;

Zhou, Lizhi ;

Chen, Tingting ;

Wang, Yingbin ;

Cheng, Tong ;

Zhang, Tianying ;

Yuan, Quan ;

Zhao, Qinjian ;

Zhang, Jun ;

McLellan, Jason S. ;

Zhou, Z. Hong ;

Zhang, Zheng ;

Li, Shaowei ;

Gu, Ying ;

Xia, Ningshao .

NATURE COMMUNICATIONS, 2021, 12 (01)

← 1 2 3 4 5 →