Effective detection of early Citrus Huanglongbing by polyethyleneimine modified multi-walled carbon nanotubes gas sensor

被引:8
作者
Weng, Jingfeng [1 ]
Zhang, Junzhe [1 ]
Zhang, Chunyu [1 ]
Lv, Jin [2 ]
Liu, Jinhui [2 ]
Zhou, Chengqian [3 ]
Yuan, Jun [4 ]
Wang, Min [4 ]
Xu, Dongqing [5 ]
Zhong, Yun [6 ]
Chen, Wenli [1 ]
机构
[1] South China Normal Univ, Inst Laser Life Sci, Coll Biophoton,Guangzhou Key Lab Spectral Analysi, MOE Key Lab Laser Life Sci,Guangdong Provincial K, Guangzhou 510631, Peoples R China
[2] South China Normal Univ, Anal & Testing Ctr, Guangzhou 510631, Peoples R China
[3] Hugo Moser Res Inst Kennedy Krieger, Neurosci Lab, Baltimore, MD 21205 USA
[4] Nanjing Agr Univ, Coll Resources & Environm Sci, Weigang 1, Nanjing 210095, Peoples R China
[5] Nanjing Agr Univ, Coll Life Sci, Weigang 1, Nanjing 210095, Peoples R China
[6] Guangdong Acad Agr Sci, Inst Fruit Tree Res,Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou 510640, Peoples R China
基金
中国国家自然科学基金;
关键词
Citrus Huanglongbing; Volatile organic compounds; Carboxylic multi-walled carbon nanotubes; Gas sensors; Polyethyleneimine; DISEASE; FABRICATION; PLATFORM;
D O I
10.1016/j.snb.2022.132508
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Citrus Huanglongbing (HLB) is incurable, so it is necessary to develop an accurate and timely detection method for citrus HLB. Volatile organic compounds (VOCs) of plants can be used as an indicator of their health status. Based on the difference of VOCs between healthy and HLB citrus leaves, we designed the polyethyleneimine (PEI)-modified carboxylic multi-walled carbon nanotubes (CNT-COOH) gas sensor and used it in the diagnosis of citrus HLB. Our PEI/CNT-COOH sensor showed significant impedance responses to four citrus HLB biomarkers VOCs (ethylhexanol, phenylacetaldehyde, linalool and methyl salicylate) with sensitivity up to single-digit ppm. We then used the sensor to detect the VOCs in citrus leaves, and found that the diseased citrus can be distin-guished from the healthy citrus according to the different response values of the sensor, successfully identified HLB-infected citrus. Compared with the conventional qPCR method, PEI/CNT-COOH sensor not only has higher accuracy, but also can be used in real time field detection of citrus leaves due to its less stringent operating condition and portability.
引用
收藏
页数:11
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