Quantitation of L-Arginine and Asymmetric Dimethylarginine in Human Plasma by LC-Selective Ion Mode-MS for Type 2 Diabetes Mellitus Study

被引：7

作者：

Yi, Lunzhao ^{[1
]}

Zhang, Pengfei ^{[2
]}

Ji, Xiaoyan ^{[1
]}

Liang, Yizeng ^{[1
]}

Wang, Yamin ^{[1
]}

Zhou, Zhiguang ^{[4
]}

Chen, Benmei ^{[3
]}

机构：

[1] Cent S Univ, Res Ctr Modernizat Chinese Herbal Med, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China

[2] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, Changsha, Hunan, Peoples R China

[3] Cent S Univ, Analyt Testing Ctr, Sch Xiangya Med, Changsha 410078, Hunan, Peoples R China

[4] Cent S Univ, Xiangya Hosp 2, Ctr Diabet, Changsha 410011, Hunan, Peoples R China

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2011年 / 59卷 / 07期

关键词：

asymmetric dimethylarginine; L-arginine type 2 diabetes mellitus; LC/MS; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; NITRIC-OXIDE SYNTHESIS; ENDOTHELIAL DYSFUNCTION; INSULIN-RESISTANCE; SYMMETRIC DIMETHYLARGININE; METHYLATED ARGININES; SYNTHASE INHIBITOR; HUMAN URINE; ADMA;

D O I：

10.1248/cpb.59.839

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The article reports a simple, sensitive and fast LC/MS method for the analysis of L-arginine (L-Arg), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) in human plasma. The homoarginine was used as the internal standard (IS). The chromatographic separation was achieved on C-18 (150 mm x 2.1 mm, 5 mu m) column with a mobile phase consisting of ammonium acetate (0.25 mmol/1) and methanol (93 : 7, v/v), at a flow rate of 0.2 ml/min. L-Arg, ADMA and SDMA were well separated by LC/MS with selective ion mode (SIM). The method was successfully applied to type 2 diabetes mellitus (T2DM) study. Twenty-one healthy controls and twenty-two T2DM patients before and after treatment two years were investigated. The results indicated that the level of ADMA in T2DM was significantly higher than that in healthy controls. Furthermore, ADMA has important association with the development of cardiovascular diseases.

引用

页码：839 / 843

页数：5

共 30 条

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