The transcriptional activators AraR and XlnR from Aspergillus niger regulate expression of pentose catabolic and pentose phosphate pathway genes

被引：33
作者：
Battaglia, Evy [1 ,2 ]
Zhou, Miaomiao [2 ]
de Vries, Ronald P. [1 ,2 ]
机构：
[1] Univ Utrecht, Microbiol & Kluyver Ctr Genom Ind Fermentat, NL-3584 CH Utrecht, Netherlands
[2] CBS Fungal Biodivers Ctr, NL-3584 CY Utrecht, Netherlands
来源：
RESEARCH IN MICROBIOLOGY | 2014年 / 165卷 / 07期
关键词：
Pentose phosphate pathway; Aspergillus niger; Regulation of gene expression; XlnR; AraR; D-XYLOSE; REDUCTASE GENE; L-ARABINOSE; METABOLISM; NORMALIZATION; VARIANCE; REVEALS;
D O I：
10.1016/j.resmic.2014.07.013
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The pentose catabolic pathway (PCP) and the pentose phosphate pathway (PPP) are required for the conversion of pentose sugars in fungi and are linked via D-xylulose-5-phosphate. Previously, it was shown that the PCP is regulated by the transcriptional activators XlnR and AraR in Aspergillus niger. Here we assessed whether XlnR and AraR also regulate the PPP. Expression of two genes, rpiA and talB, was reduced in the Delta araR/Delta xlnR strain and increased in the xylulokinase negative strain (xkiA1) on D-xylose and/or L-arabinose. Bioinformatic analysis of the 1 kb promoter regions of rpiA and talB showed the presence of putative XlnR binding sites. Combining all results in this study, it strongly suggests that these two PPP genes are under regulation of XlnR in A. niger. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：531 / 540
页数：10
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