Molecular Markers for Identifying Resistance Genes in Brassica napus

被引：7

作者：

Van de Wouw, Angela P. ^{[1
]}

Zhang, Yueqi ^{[2
]}

Saad, Nur Shuhadah Mohd ^{[2
]}

Yang, Hua ^{[2
]}

Sheedy, Elizabeth ^{[3
]}

Elliott, Candace E. ^{[1
]}

Batley, Jacqueline ^{[2
]}

机构：

[1] Univ Melbourne, Sch BioSci, Melbourne, Vic 3010, Australia

[2] Univ Western Australia, Sch Biol Sci, Crawley, WA 6009, Australia

[3] Grains Innovat Pk, Marcroft Grains Pathol, Horsham, Vic 3400, Australia

来源：

AGRONOMY-BASEL | 2022年 / 12卷 / 05期

关键词：

Kompetitive Allele-Specific PCR (KASP); blackleg; Leptosphaeria maculans; canola; molecular breeding; LEPTOSPHAERIA-MACULANS; BLACKLEG RESISTANCE; OILSEED RAPE; AVIRULENCE; DISEASE; IDENTIFICATION; AVRLM4-7; CANOLA; CROPS;

D O I：

10.3390/agronomy12050985

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is the most devastating disease of canola (Brassica napus, oilseed rape) worldwide. Breeding for genetic resistance is the most widely used tool for controlling this disease and minimizing the impact on yield. To date, five resistance genes (Rlm2, LepR3, Rlm4, Rlm7, Rlm9) have been cloned from B. napus, representing alleles of two different gene loci, Rlm2-LepR3 and Rlm4-7-9. We report on the development and validation of Kompetitive Allele-Specific PCR (KASP) markers that can discriminate between the resistant and susceptible alleles of each resistance gene. These markers will provide valuable tools for both researchers and industry through the ability to characterize resistance genes without phenotyping.

引用

页数：13

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