Study on the Interaction of DNA with Resveratrol by Resonance Light Scattering Technique and Its Analytical Application

被引：3

作者：

Xiang, Haiyan ^{[1
]}

Xie, Yang ^{[1
]}

Huang, Jianding ^{[1
]}

机构：

[1] So Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Guangdong, Peoples R China

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2011年 / 66卷 / 06期

关键词：

DNA; resveratrol; resonance light scattering technique; NUCLEIC-ACIDS; CETYLTRIMETHYLAMMONIUM BROMIDE; ION;

D O I：

10.1134/S1061934811040071

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The interaction between resveratrol and DNA has been studied by resonance light scattering (RLS) technique. In strongly acidic solution, resveratrol has a maximum peak at 368 nm and the RLS intensity is remarkably enhanced by trace amounts of DNA due to its interaction with resveratrol. Based on this, a novel assay for nucleic acids has been developed. The characteristics of RLS, fluorescence and UV-VIS absorption spectra, the influential factors and optimum conditions of the reaction have been studied. The enhanced RLS intensity at 368 nm is proportional to the concentration of DNA within the range of 0-1600 mu g/L for calf thymus DNA. The determination limit (3 sigma) is 5.2 ng/mL. The study of foreign substance effect on the determination of DNA indicates that most of metal ions have little effect on the determination of DNA. Three synthetic samples of DNA were analysed with satisfactory results. The results show that the proposed method is very sensitive, convenient, rapid and reproducible.

引用

页码：618 / 622

页数：5

共 17 条

[1] Therapeutic potential of resveratrol:: the in vivo evidence [J].

Baur, Joseph A. ;

Sinclair, David A. .

NATURE REVIEWS DRUG DISCOVERY, 2006, 5 (06) :493-506

[2] Spectral studies on the interaction of Acid chrome blue K with nucleic acids in the presence of cetyltrimethylammonium bromide and the confirmation of combined points [J].

Cai, Changqun ;

Chen, Xiaoming .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2008, 69 (02) :592-598

[3] Interaction of anticancer drug methotrexate with nucleic acids analyzed by multi-spectroscopic method [J].

Cai, Changqun ;

Chen, Xiaoming ;

Gong, Hang .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2009, 72 (01) :46-49

[4] Application of functional CdS nanoparticles in determination of silver ion by resonance light-scattering technique [J].

Chen, Hong-Qi ;

Luo, Fa-Bo ;

Liu, Yan ;

Liang, A. -Ni ;

Lin, Bo ;

Wang, Lun .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2009, 71 (05) :1701-1703

[5] Resonance light scattering spectroscopy study of interaction between norfloxacin and calf thymus DNA and its analytical application [J].

Chen, Zhanguang ;

Zhang, Taiyu ;

Han, Yali ;

Zhu, Li .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2006, 65 (3-4) :919-924

[6] A novel curcumin assay with the metal ion Cu (II) as a simple probe by resonance light scattering technique [J].

Chen, Zhanguang ;

Zhu, Li ;

Song, Tianhe ;

Chen, Junhui ;

Guo, Zhiming .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2009, 72 (03) :518-522

[7] Investigation on hyperin-cetyltrimethylammonium bromide-fibronectin system by resonance light-scattering technique [J].

Gao, Dejiang ;

Tian, Yuan ;

Liang, Fanghui ;

Bi, Shuyun ;

Li, Tiechun ;

Chen, Yanhua ;

Zhang, Hanqi ;

Yu, Aimin .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2007, 66 (01) :52-57

[8] Simple and sensitive determination of nucleic acids using palladium(II) complex with 2-(2-thiazolylazo)-5-dimethylaminobenzoic acid [J].

Hao, YM ;

Shen, HX .

ANALYTICA CHIMICA ACTA, 2000, 422 (02) :159-166

[9] Determination of nucleic acids by a resonance light-scattering technique with alpha,beta,gamma,delta-tetrakis[4-(trimothylammoniumyl)phenyl]porphine [J].

Huang, CZ ;

Li, KA ;

Tong, SY .

ANALYTICAL CHEMISTRY, 1996, 68 (13) :2259-2263

[10] Determination of nucleic acid based on increased resonance light-scattering of fluorinated surfactants [J].

Li, Ling ;

Xu, Zu Shun ;

Pan, Quan ;

Song, Gong Wu .

JOURNAL OF FLUORINE CHEMISTRY, 2009, 130 (06) :567-572

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