The splice is right: Guarantors of fidelity in pre-mRNA splicing

被引：16
作者：
Horowitz, David S. [1 ]
机构：
[1] Uniformed Serv Univ Hlth Sci, Dept Biochem & Mol Biol, Bethesda, MD 20814 USA
来源：
RNA | 2011年 / 17卷 / 04期
关键词：
Prp16; splicing fidelity; RNA helicase; DEXD/H-BOX ATPASE; DEPENDENT ATPASE; CONFORMATIONAL REARRANGEMENT; PRP16; STEP; COOPERATE; PROOFREAD; INTERACTS; MUTANTS; RELEASE;
D O I：
10.1261/rna.2577511
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two recent papers, one from the Staley laboratory (Koodathingal and colleagues) and the other from the Cheng laboratory (Tseng and colleagues), show that the RNA-dependent ATPase Prp16, which is required for the second step of splicing, acts to reject slowly splicing pre-mRNAs immediately before the first catalytic reaction in pre-mRNA splicing. The results answer long-investigated questions about the actions of Prp16 and provide a wealth of molecular details on the proofreading process in pre-mRNA splicing. The discussion here reviews and integrates the results of the two papers and describes the implications for proofreading in splicing.
引用
收藏
页码：551 / 554
页数：4
相关论文
共 23 条
  • [1] Prp43: An RNA helicase-like factor involved in spliceosome disassembly
    Arenas, JE
    Abelson, JN
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (22) : 11798 - 11802
  • [2] A MECHANISM TO ENHANCE MESSENGER-RNA SPLICING FIDELITY - THE RNA-DEPENDENT ATPASE PRP16 GOVERNS USAGE OF A DISCARD PATHWAY FOR ABERRANT LARIAT INTERMEDIATES
    BURGESS, SM
    GUTHRIE, C
    [J]. CELL, 1993, 73 (07) : 1377 - 1391
  • [3] Cwc25 Is a Novel Splicing Factor Required after Prp2 and Yju2 To Facilitate the First Catalytic Reaction
    Chiu, Ying-Fang
    Liu, Yen-Chi
    Chiang, Ting-Wei
    Yeh, Tzu-Chi
    Tseng, Chi-Kang
    Wu, Nan-Ying
    Cheng, Soo-Chen
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2009, 29 (21) : 5671 - 5678
  • [4] A TRANS-ACTING SUPPRESSOR RESTORES SPLICING OF A YEAST INTRON WITH A BRANCH POINT MUTATION
    COUTO, JR
    TAMM, J
    PARKER, R
    GUTHRIE, C
    [J]. GENES & DEVELOPMENT, 1987, 1 (05) : 445 - 455
  • [5] The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome
    Fabrizio, Patrizia
    Dannenberg, Julia
    Dube, Prakash
    Kastner, Berthold
    Stark, Holger
    Urlaub, Henning
    Luehrmann, Reinhard
    [J]. MOLECULAR CELL, 2009, 36 (04) : 593 - 608
  • [6] How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing
    James, SA
    Turner, W
    Schwer, B
    [J]. RNA, 2002, 8 (08) : 1068 - 1077
  • [7] The DEAH Box ATPases Prp16 and Prp43 Cooperate to Proofread 5′ Splice Site Cleavage during Pre-mRNA Splicing
    Koodathingal, Prakash
    Novak, Thaddeus
    Piccirilli, Joseph A.
    Staley, Jonathan P.
    [J]. MOLECULAR CELL, 2010, 39 (03) : 385 - 395
  • [8] Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing
    Lardelli, Rea M.
    Thompson, James X.
    Yates, John R., III
    Stevens, Scott W.
    [J]. RNA, 2010, 16 (03) : 516 - 528
  • [9] A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing
    Liu, Yen-Chi
    Chen, Hsin-Chou
    Wu, Nan-Ying
    Cheng, Soo-Chen
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (15) : 5403 - 5413
  • [10] MADHANI HD, 1994, GENETICS, V137, P677
123