Purification of polysaccharides from Cordyceps militaris and their anti-hypoxic effect

被引:21
作者
Dong, Yuan [1 ,2 ]
Hu, Shuang [1 ]
Liu, Chungang [1 ]
Meng, Qingfan [1 ]
Song, Jia [1 ]
Lu, Jiahui [1 ]
Cheng, Yingkun [1 ]
Gao, Chaohui [1 ]
Liu, Yan [1 ]
Wang, Di [1 ]
Teng, Lirong [1 ,3 ]
机构
[1] Jilin Univ, Coll Life Sci, Changchun 130012, Jilin, Peoples R China
[2] Jilin Med Coll, Acad Lab, Changchun 132013, Jilin, Peoples R China
[3] Jilin Univ, Zhuhai Coll, Coll Life Sci, Zhuhai 519000, Guangdong, Peoples R China
关键词
Cordyceps militaris; polysaccharides; purification; structural analysis; anti-hypoxic effect; ANTI-HYPOXIA ACTIVITY; STRUCTURAL-CHARACTERIZATION; HYPOGLYCEMIC ACTIVITY; ALTITUDE; ACTIVATION; EXPRESSION; SINENSIS;
D O I
10.3892/mmr.2014.2786
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Acute mountain sickness, one of the most common altitude diseases, causes lung and brain injury. The present study aimed to investigate the anti-hypoxic effect of purified polysaccharides extracted from Cordyceps militaris. The aqueous extract of Cordyceps militaris was purified progressively through a DEAE-52 cellulose anion exchange column and a Sepharose G-100 column. The fraction CMN1, with a molecular weight of 37842 Da, was the main fraction obtained and its chemical composition and structural characteristics were determined. CMN1 was found to have a monosaccharide composition of L-rhamnose, L-arabinose, D-mannose, D-galactose. The backbone of CMN1 comprised (1 -> 2) and (1 -> 3) linkages, with branched (1 -> 6) and (1 -> 4) linkages. The anti-hypoxic effects of CMN1 were determined using a sodium nitrite toxicosis test, acute cerebral ischemic/hypoxic test and normobarie hypoxia test. CMN1 (0.5 g/kg) possessed a similar anti-hypoxic effect to rhodiola oral liquid. Overall, the Cordyceps militaris polysaccharide, CMN1, was identified as an effective agent against hypoxia.
引用
收藏
页码:1312 / 1317
页数:6
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