Human Umbilical Cord Blood Therapy Protects Cerebral White Matter from Systemic LPS Exposure in Preterm Fetal Sheep

被引:39
作者
Paton, Madison C. B. [1 ,2 ]
Allison, Beth J. [1 ]
Li, Jingang [1 ]
Fahey, Michael C. [1 ,3 ]
Sutherland, Amy E. [1 ]
Nitsos, Ilias [1 ]
Bischof, Robert J. [1 ]
Dean, Justin M. [4 ]
Moss, Timothy J. M. [1 ,2 ]
Polglase, Graeme R. [1 ,2 ]
Jenkin, Graham [1 ,2 ]
McDonald, Courtney A. [1 ]
Miller, Suzanne L. [1 ,2 ]
机构
[1] Hudson Inst Med Res, Ritchie Ctr, Clayton, Vic, Australia
[2] Monash Univ, Dept Obstet & Gynaecol, Clayton, Vic, Australia
[3] Monash Hlth, Dept Paediat, Monash Childrens, Clayton, Vic, Australia
[4] Univ Auckland, Dept Physiol, Auckland, New Zealand
基金
英国医学研究理事会; 澳大利亚研究理事会;
关键词
Brain injury; Cerebral palsy; Fetal sheep; Infection; Inflammation; Neonatal neuroinflammation; Stem cells; White matter; White matter damage; BRAIN-INJURY; PERINATAL ASPHYXIA; CELLS; CHORIOAMNIONITIS; PALSY; NEUROINFLAMMATION; MICROGLIA; CYTOKINES; DAMAGE;
D O I
10.1159/000490943
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Infants born preterm following exposure to in utero inflammation/chorioamnionitis are at high risk of brain injury and life-long neurological deficits. In this study, we assessed the efficacy of early intervention umbilical cord blood (UCB) cell therapy in a large animal model of preterm brain inflammation and injury. We hypothesised that UCB treatment would be neuroprotective for the preterm brain following subclinical fetal inflammation. Methods: Chronically instrumented fetal sheep at 0.65 gestation were administered lipopolysaccharide (LPS, 150 ng, 055:B5) intravenously over 3 consecutive days, followed by 100 million human UCB mononuclear cells 6 h after the final LPS dose. Controls were administered saline instead of LPS and cells. Ten days after the first LPS dose, the fetal brain and cerebrospinal fluid were collected for analysis of subcortical and periventricular white matter injury and inflammation. Results: LPS administration increased microglial aggregate size, neutrophil recruitment, astrogliosis and cell death compared with controls. LPS also reduced total oligodendrocyte count and decreased mature myelinating oligodendrocytes. UCB cell therapy attenuated cell death and inflammation, and recovered total and mature oligodendrocytes, compared with LPS. Conclusions: UCB cell treatment following inflammation reduces preterm white matter brain injury, likely mediated via anti-inflammatory actions. (C) 2018 S. Karger AG, Basel
引用
收藏
页码:258 / 270
页数:13
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