Persister Cells Resuscitate Using Membrane Sensors that Activate Chemotaxis, Lower cAMP Levels, and Revive Ribosomes

被引:61
作者
Yamasaki, Ryota [1 ]
Song, Sooyeon [1 ]
Benedik, Michael J. [3 ]
Wood, Thomas K. [1 ,2 ]
机构
[1] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA
[2] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA
[3] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA
关键词
AMP RECEPTOR PROTEIN; ESCHERICHIA-COLI; CYCLIC-AMP; BACTERIAL-INFECTIONS; IDENTIFICATION; INCREASES; PHOSPHODIESTERASE; TOLERANCE; LIBRARY; GENES;
D O I
10.1016/j.isci.2019.100792
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Persistence, the stress-tolerant state, is arguably the most vital phenotype since nearly all cells experience nutrient stress, which causes a sub-population to become dormant. However, how persister cells wake to reconstitute infections is not understood well. Here, using single-cell observations, we determined that Escherichia coli persister cells resuscitate primarily when presented with specific carbon sources, rather than spontaneously. In addition, we found that the mechanism of persister cell waking is through sensing nutrients by chemotaxis and phosphotransferase membrane proteins. Furthermore, nutrient transport reduces the level of secondary messenger cAMP through enzyme IIA; this reduction in cAMP levels leads to ribosome resuscitation and rescue. Resuscitating cells also immediately commence chemotaxis toward nutrients, although flagellar motion is not required for waking. Hence, persister cells wake by perceiving nutrients via membrane receptors that relay the signal to ribosomes via the secondary messenger CAMP, and persisters wake and utilize chemotaxis to acquire nutrients.
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页数:66
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