Behaviour of isolated rat and human red blood cells upon hypotonic-dialysis encapsulation of carbonic anhydrase and dextran

被引：0

作者：

Alvarez, FJ ^{[1
]}

Herraez, A ^{[1
]}

Tejedor, MC ^{[1
]}

Diez, JC ^{[1
]}

机构：

[1] UNIV ALCALA DE HENARES, DEPT BIOQUIM & BIOL MOLEC, E-28871 ALCALA DE HENARES, MADRID, SPAIN

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 1996年 / 23卷

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat and human carrier red blood cells (RBCs) were prepared by hypotonic-dialysis encapsulation, The standard conditions used for encapsulation were 80 mOsm/kg for 60 min. The encapsulation behaviour of rat and human RBCs was studied using radiolabelled carbonic anhydrase and fluorescently labelled dextran, Both markers are incorporated to slightly greater extents by human than by rat RBCs by hypotonic treatment. Cell recovery of rat and human RBCs loaded with either carbonic anhydrase or fluorescent dextran accounted for 49% and 80% respectively, The cellular integrity of the loaded cells was revealed by the presence of fluorescence labelling in rat and human RBCs, Fluorescence studies showed an increase of size dispersion in loaded rat and human RBCs, giving cellular volume variations in both types of cells resulting from the encapsulation procedure. Two loaded cell populations were evident in both species, one with high fluorescence content and another with background staining, Apparently the proportion of high fluorescently labelled loaded cells was higher in the case of the human RBCs, A reduced level of fluorescence labelling was observed in rat and human RBC membranes, which indicates a process of adsorption of dextran to the membranes.

引用

页码：173 / 179

页数：7

共 30 条

[1] MODIFICATION OF PARTIAL-PRESSURE OF OXYGEN (P-50) IN MAMMALIAN RED BLOOD-CELLS BY INCORPORATION OF AN ALLOSTERIC EFFECTOR OF HEMOGLOBIN [J].

BAILLEUL, C ;

BORRELLYVILLEREAL, MO ;

CHASSAIGNE, M ;

ROPARS, C .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1989, 11 (01) :31-40

[2]

CHIARANTINI L, 1991, BLOOD CELLS, V17, P607

[3]

DEFLORA A, 1986, P NATL ACAD SCI USA, V83, P7029

[4] CARRIER ERYTHROCYTES FROM CATTLE CAN BE PREPARED FROM CELLS STORED IN A HYPOTONIC, POROUS, DIALYZED STATE [J].

DELOACH, JR ;

ANDREWS, K .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1987, 9 (06) :443-449

[5]

DELOACH JR, 1990, BIOTECHNOL APPL BIOC, V12, P331

[6]

DELOACH JR, 1987, METHOD ENZYMOL, V149, P235

[7]

DELOACH JR, 1988, BIOTECHNOL APPL BIOC, V10, P154

[8] CARRIER ERYTHROCYTES [J].

DELOACH, JR .

MEDICINAL RESEARCH REVIEWS, 1986, 6 (04) :487-504

[9]

DELOACH JR, 1988, BIOTECHNOL APPL BIOC, V10, P183

[10]

DELOACH JR, 1993, BIOTECHNOL APPL BIOC, V18, P83

← 1 2 3 →