Sex bias miRNAs in Cynoglossus semilaevis could play a role in transgenerational inheritance

Alterations of non-coding RNA profiling in spermatozoa are candidate mechanisms related to changes in paternal environment and progeny. Transgenerational inheritance of sex in pseudomales of Cynoglossus semilaevis, a fish with significant sex dimorphism, is a typical example of non-Mendelian inheritance. In the present study, miRNA profiles of spermatozoa were compared between male and pseudomale of C. semilaevis. Differential miRNAs in sperm from F0 and F1 generation also provides clues for revealing the possible role of non-coding RNA mediated transgenerational inheritance. Four sexual bias miRNAs, dre-miR-26a-5p, dre-miR-27b-3p, dre-miR-125b-5p,pol199a-5p, were identified and verified in F0 and F1 generation of C. semilaevis. All of them were highly expressed in male sperm compared with pseudomale sperm. Function of target genes indicates that target genes of these differential RNAs are highly correlated with sex differentiation, gametogenesis and maintenance of secondary sexual characteristics. In a word, identification of epigenetic markers in gametes has great prospects in predicting susceptibility and properties in offsprings, and providing an indicator of parentalgenetic property.