Formate dehydrogenase gene of Arabidopsis thaliana is induced by formaldehyde and not by formic acid

被引:11
|
作者
Fukusaki, EI [1 ]
Ikeda, T [1 ]
Shiraishi, T [1 ]
Nishikawa, T [1 ]
Kobayashi, A [1 ]
机构
[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan
关键词
FDH; formate; arabidopsis; formaldehyde;
D O I
10.1263/jbb.90.691
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Variability of expression of formate dehydrogenase (FDH) caused by uptake of C-1 compounds was examined by using Arabidopsis thaliana as a model plant. Effects of uptake of several C-1 compounds were evaluated by Northern blot analysis using cDNA of A. thaliana FDH prepared by cloning on the basis of known sequence. As a result, expression of the FDH gene in A. thaliana was not intensely influenced by formic acid, an inherent substrate for FDH, but strongly induced by its reduced form, formaldehyde.
引用
收藏
页码:691 / 693
页数:3
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