Rotavirus A among hospitalized infants, up to three years of age, with acute gastroenteritis in Campo Grande, State of Mato Grosso do Sul

Polyacrylainiaegel electropboresis and combined immunoenzyme assay for rotavirus and adenovirus were used to analyze 380 fecal samples from children up to three years of age who were bospitalized with acute diarrhea in Campo Grande, State of Mato Grosso do Sul, between,May 2000 and januag 2004. Among all the samples, 88 (23.29,.) were positive for RotavirusA. Out o these, 81 (92%) had a defined electropboreticpatteni: 77 If (875%) with a longpattern andfour (4.5%) with a sbortpattern. Genotjpe G andP characterization was done by nested RT-PCR for 85 samples, of which 56 (65.9%) were genotjped as type G. Among these, 49 (87.5%) were G1, five (8.991.') were G4, one (1. 801.) was G3 and one (I. 811') was G9. Av genot)pe wasfound to be t)pe P in 37 samples (43.5%) and all of these were P[8]. The G and P association most observed was GIP[81, with 33 samples (89.2%), followed by G4P[81, two samples (5.4%); G31`18], one sample (2.7%); and G91'[81, one sample (2.7%).