Purkinje cell calcium dysregulation is the cellular mechanism that underlies catecholaminergic polymorphic ventricular tachycardia

被引：65

作者：

Herron, Todd J. ^{[1
,2
]}

Milstein, Michelle L. ^{[1
,2
]}

Anumonwo, Justus ^{[1
,2
]}

Priori, Silvia G. ^{[3
,4
]}

Jalife, Jose ^{[1
,2
]}

机构：

[1] Univ Michigan, Ctr Arrhythmia Res, Dept Internal Med, Ann Arbor, MI 48108 USA

[2] Univ Michigan, Ctr Arrhythmia Res, Dept Mol & Integrat Physiol, Ann Arbor, MI 48108 USA

[3] NYU, Dept Med, Leon H Charney Div Cardiol, New York, NY 10016 USA

[4] Univ Pavia, Mol Cardiol Fondaz S Maugeri IRCCS, I-27100 Pavia, Italy

来源：

HEART RHYTHM | 2010年 / 7卷 / 08期

关键词：

Inherited arrhythmias; Ryanodine receptor; Calcium; Cardiac Purkinje cells; Molecular mechanisms of arrhythmia; Ventricular cardiac myocytes; CA2+ TRANSIENTS; MUTATIONS; SYSTEM; WAVES; GENE;

D O I：

10.1016/j.hrthm.2010.06.010

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

BACKGROUND Inherited arrhythmias can be caused by mutations in the cardiac ryanodine receptor (RyR2). The cellular source of these arrhythmias is unknown. Isolated RyR2(R4496C) mouse ventricular myocytes display arrhythmogenic activity related to spontaneous Ca2+ release during diastole. On the other hand, recent whole-heart epicardial and endocardial optical mapping data demonstrate that ventricular arrhythmias in the RyR2(R4496C) mouse model of catecholaminergic polymorphic ventricular tachycardia (CPVT) originate in the His-Purkinje system, suggesting that Purkinje cells, and not ventricular myocytes, may be the cellular source of arrhythmogenic activity. The relative effect of the RyR2(R4496C) mutation on calcium homeostasis in ventricular myocytes versus Purkinje cells is unknown. OBJECTIVE This study sought to determine which cardiac cell type is more severely affected, in terms of calcium handling, by expression of the RyR2(R4496C) mutant channel: the ventricular myocytes or the Purkinje cells. METHODS AND RESULTS To discriminate Purkinje cells from ventricular myocytes, we crossed the RyR2(R4496C) mouse model of CPVT with the Cx40(EGFP/+) transgenic mouse. This genetic cross yields Purkinje cells that express eGFP, and therefore fluoresce green when excited by the appropriate wavelength; ventricular myocytes, which do not express connexin 40, are not green. Intracellular calcium was measured in each cell type using calcium-sensitive probes. Purkinje cells of the RyR2(R4496C) mouse model of CPVT show an approximately 2x greater rate (P < .05) and approximately 2x to 3x greater amplitude (P < .000001) of spontaneous calcium release events than ventricular myocytes isolated from the same heart. CONCLUSION These results demonstrate that focally activated arrhythmias originate in the specialized electrical conducting cells of the His-Purkinje system in the RyR2(R4496C) mouse model of CPVT.

引用

页码：1122 / 1128

页数：7

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