Fluroxypyr- and triclopyr-specific enzyme-linked immunosorbent assays: Development and quantitation in soil and water

被引：24

作者：

Johnson, BD ^{[1
]}

Hall, JC ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT ENVIRONM BIOL,GUELPH,ON N1G 2W1,CANADA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1996年 / 44卷 / 02期

关键词：

ELISA; residue; pyridine; herbicide;

D O I：

10.1021/jf9405867

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Enzyme-linked immunosorbent assays (ELISAs) were developed to quantitate fluroxypyr [[(4-amino-3, 5-dichloro-6-fluoro-2-pyridinyl)oxy] acetic acid] and triclopyr [[(3,5,6-trichloro-2-pyridinyl)oxy] acetic acid] in soil and water. The linear working range of the fluroxypyr ELISA was from 0.1 to 10 ng . mL(-1) with a limit of detection (LOD) of 0.1 ng . mL(-1) and an IC50 value of 1.6 ng . mL(-1). The linear working range of the triclopyr ELISA was from 0.1 to 5 ng . mL(-1) with a LOD of 0.1 ng . mL(-1) and an IC50 value of 1.7 ng . mL(-1). Cross-reactivity to selected pyridine metabolites and agrochemicals was determined. Significant cross-reactivity (within the linear working range of the ELISA) using the fluroxypyr ELISA was found only to the metabolite 4-amino-3, 5-dichloro-6-fluoro-2-methoxypyridine. Significant cross-reactivity using the triclopyr ELISA was found only to the auxinic herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The ELISAs accurately estimated fluroxypyr and triclopyr in water at concentrations as low as 0.1 ng . mL(-1). Analysis of two different soil types with different textures (clay loam and sandy clay loam) required cleanup procedures using filtration and solid phase extraction to accurately estimate fluroxypyr and triclopyr concentrations.

引用

页码：488 / 496

页数：9

共 44 条

[1] LEACHING POTENTIAL AND DECOMPOSITION OF FLUROXYPYR IN SWEDISH SOILS UNDER FIELD CONDITIONS [J].

BERGSTROM, LF ;

MCGIBBON, AS ;

DAY, SR ;

SNEL, M .

PESTICIDE SCIENCE, 1990, 29 (04) :405-417

[2] BOUND WATER-MOLECULES AND CONFORMATIONAL STABILIZATION HELP MEDIATE AN ANTIGEN-ANTIBODY ASSOCIATION [J].

BHAT, TN ;

BENTLEY, GA ;

BOULOT, G ;

GREENE, MI ;

TELLO, D ;

DALLACQUA, W ;

SOUCHON, H ;

SCHWARZ, FP ;

MARIUZZA, RA ;

POLJAK, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (03) :1089-1093

[3]

BUSHWAY RJ, 1989, J AGR FOOD CHEM, V37, P647

[4]

Butler J. E., 1993, Molecular Immunology, V30, P1165, DOI 10.1016/0161-5890(93)90135-X

[5]

CAMPBELL AM, 1984, MONOCLONAL ANTIBODY, V1

[6] CRYSTALLOGRAPHIC REFINEMENT AND ATOMIC MODELS OF A HUMAN FC FRAGMENT AND ITS COMPLEX WITH FRAGMENT-B OF PROTEIN-A FROM STAPHYLOCOCCUS-AUREUS AT 2.9-A AND 2.8-A RESOLUTION [J].

DEISENHOFER, J .

BIOCHEMISTRY, 1981, 20 (09) :2361-2370

[7]

DESCHAMPS R J A, 1991, Food and Agricultural Immunology, V3, P135, DOI 10.1080/09540109109354740

[8] POLYCLONAL AND MONOCLONAL ENZYME IMMUNOASSAYS FOR PICLORAM DETECTION IN WATER, SOIL, PLANTS, AND URINE [J].

DESCHAMPS, RJA ;

HALL, JC ;

MCDERMOTT, MR .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1990, 38 (09) :1881-1886

[9] ORGANIC-PHOTOCHEMISTRY .19. QUANTUM YIELDS FOR O,O-DIETHYL O-(3,5,6-TRICHLORO-2-PYRIDINYL) PHOSPHOROTHIOATE (CHLORPYRIFOS) AND 3,5,6-TRICHLORO-2-PYRIDINOL IN DILUTE AQUEOUS-SOLUTIONS AND THEIR ENVIRONMENTAL PHOTOTRANSFORMATION RATES [J].

DILLING, WL ;

LICKLY, LC ;

LICKLY, TD ;

MURPHY, PG ;

MCKELLAR, RL .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1984, 18 (07) :540-543

[10] DEVELOPMENT OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR ALACHLOR AND ITS APPLICATION TO THE ANALYSIS OF ENVIRONMENTAL WATER SAMPLES [J].

FENG, PCC ;

WRATTEN, SJ ;

HORTON, SR ;

SHARP, CR ;

LOGUSCH, EW .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1990, 38 (01) :159-163

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