Cryo-staining techniques in cryo-TEM studies of dispersed nanotubes

被引：12

作者：

Edri, Eran ^{[1
]}

Regev, Oren ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Dept Chem Engn, IL-84105 Beer Sheva, Israel

来源：

ULTRAMICROSCOPY | 2010年 / 110卷 / 07期

关键词：

Staining; Positive; Negative; Cryo-TEM; Nanotube; Dispersion; WALLED CARBON NANOTUBES; THREAD-LIKE MICELLES; ELECTRON-MICROSCOPY; AQUEOUS-SOLUTIONS; LIGHT-SCATTERING; DRUG-DELIVERY; SERUM-ALBUMIN; POLYMERS; MECHANISM; PROTEINS;

D O I：

10.1016/j.ultramic.2010.03.010

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The combination of cryo-TEM and staining is employed for studying protein-enabled dispersion of carbon nanotubes in aqueous solution. The same staining agent is used for both positive- and negative-staining. We are able to image the adsorbed layer of protein or polysaccharide on the nanotube but not the individual molecule. The process is not artifact-free due to change in ionic strength of the solution. However, our results are in line with other, not related, experimental techniques. The obtained information could be used to update models suggested based on, e.g., scattering data. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：751 / 757

页数：7

共 43 条

[1] Cryo-negative staining [J].