Titanium particles induce apoptosis by promoting autophagy in macrophages via the PI3K/Akt signaling pathway

被引:14
|
作者
Xian, Guoyan [1 ]
Chen, Weishen [1 ]
Gu, Minghui [1 ]
Ye, Yongyu [1 ]
Yang, Guangpu [1 ]
Lai, Weiming [1 ]
Xiao, Yinbo [2 ]
Zhao, Xiaoyi [1 ]
Zheng, Linli [1 ]
Pan, Baiqi [1 ]
Kang, Yunze [1 ]
Zhang, Ziji [1 ]
Sheng, Puyi [1 ]
机构
[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Joint Surg, 58 Zhongshan 2nd Rd, Guangzhou 510080, Peoples R China
[2] Univ Glasgow, Coll Med Vet & Life Sci, Inst Mol Cell & Syst Biol, Ctr Cellular Microenvironm, Glasgow, Lanark, Scotland
基金
中国国家自然科学基金;
关键词
apoptosis; autophagy; macrophages; periprosthetic joint infection; wear particles; PERI-IMPLANT OSTEOLYSIS; NF-KAPPA-B; WEAR PARTICLES; CELL-DEATH; INFLAMMATION; POLYETHYLENE; EXPRESSION;
D O I
10.1002/jbm.a.36938
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Chronic inflammation and infection in the tissue surrounding implants after total joint replacement is closely associated with the innate immune response to surgical implants. Wear particles are known to increase apoptosis and impair the innate immunity in macrophages, which can cause immunosuppression around the implants. Excessive autophagy can induce apoptosis. However, the link between autophagy and apoptosis in macrophages during chronic inflammation and infection remains unknown. In this study, we investigated the autophagy and apoptosis induced by titanium particles in RAW264.7 macrophages, and in the interface membrane of patients with late-onset periprosthetic joint infection (PJI). We found that titanium particles stimulated autophagy and apoptosis in macrophages. Inhibition of autophagy significantly reduced titanium particle-induced apoptosis in macrophages, which may be related to the PI3K/Akt signaling pathway. The secretion of inflammatory factors, such as IL-1 beta, IL-6, and TNF-alpha, decreased after inhibition of autophagy in titanium particle-stimulated macrophages, which may be caused by immune dysfunction due to titanium particle-induced autophagy and apoptosis in macrophages. Furthermore, our in vivo mouse calvarial model also showed that autophagy inhibitors lowered the rate of cell apoptosis. Our findings indicate that wear particle-induced apoptosis may be caused by enhanced autophagy in macrophages, which could potentially impair the local innate immunity in periprosthetic tissues and could be a risk factor for PJI. Based on these results, autophagy modulators may act as a new therapeutic option for PJI.
引用
收藏
页码:1792 / 1805
页数:14
相关论文
共 50 条
  • [1] Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway
    Duan, Junchao
    Yu, Yongbo
    Yu, Yang
    Wang, Ji
    Geng, Weijia
    Jiang, Lizhen
    Li, Qiuling
    Zhou, Xianqing
    Sun, Zhiwei
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2014, 9 : 5131 - 5141
  • [2] Physalin A Induces Apoptosis and Autophagy in Hepatocellular Carcinoma via Inhibition of PI3K/Akt Signaling Pathway
    Shang, Xiang
    Chen, Zhen
    Liu, Jianxiong
    Xu, Shuguo
    Li, Longbiao
    Yang, Zhihong
    Cui, Yuansheng
    Ruan, Pingzhao
    Peng, Xiaolan
    BIOCHEMICAL GENETICS, 2024, 62 (02) : 633 - 644
  • [3] Zinc oxide nanoparticles induce apoptosis by enhancement of autophagy via PI3K/Akt/mTOR inhibition
    Roy, Ruchi
    Singh, Sunil Kumar
    Chauhan, K. S.
    Das, Mukul
    Tripathi, Anurag
    Dwivedi, Premendra D.
    TOXICOLOGY LETTERS, 2014, 227 (01) : 29 - 40
  • [4] Parthenolide induces apoptosis and autophagy through the suppression of PI3K/Akt signaling pathway in cervical cancer
    Jeyamohan, Sridharan
    Moorthy, Rajesh Kannan
    Kannan, Mahesh Kumar
    Arockiam, Antony Joseph Velanganni
    BIOTECHNOLOGY LETTERS, 2016, 38 (08) : 1251 - 1260
  • [5] Dibenzoxanthenes induce apoptosis and autophagy in HeLa cells by modeling the PI3K/Akt pathway
    Wang, Xiu-Zhen
    Jia, Zhi
    Yang, Hui-Hui
    Liu, Yun-Jun
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2018, 187 : 76 - 88
  • [6] Physalin A Induces Apoptosis and Autophagy in Hepatocellular Carcinoma via Inhibition of PI3K/Akt Signaling Pathway
    Xiang Shang
    Zhen Chen
    Jianxiong Liu
    Shuguo Xu
    Longbiao Li
    Zhihong Yang
    Yuansheng Cui
    Pingzhao Ruan
    Xiaolan Peng
    Biochemical Genetics, 2024, 62 : 633 - 644
  • [7] Lycopene attenuates high glucose-mediated apoptosis in MPC5 podocytes by promoting autophagy via the PI3K/AKT signaling pathway
    Wang, Qingfen
    Li, Rui
    Xiao, Zhi
    Hou, Cun
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2020, 20 (03) : 2870 - 2878
  • [8] Deoxynivalenol induces apoptosis and autophagy in human prostate epithelial cells via PI3K/Akt signaling pathway
    Kowalska, Karolina
    Koziel, Marta Justyna
    Habrowska-Gorczynska, Dominika Ewa
    Urbanek, Kinga Anna
    Dominska, Kamila
    Piastowska-Ciesielska, Agnieszka Wanda
    ARCHIVES OF TOXICOLOGY, 2022, 96 (01) : 231 - 241
  • [9] Deoxynivalenol induces apoptosis and autophagy in human prostate epithelial cells via PI3K/Akt signaling pathway
    Karolina Kowalska
    Marta Justyna Kozieł
    Dominika Ewa Habrowska-Górczyńska
    Kinga Anna Urbanek
    Kamila Domińska
    Agnieszka Wanda Piastowska-Ciesielska
    Archives of Toxicology, 2022, 96 : 231 - 241
  • [10] Eupafolin ameliorates lipolysaccharide-induced cardiomyocyte autophagy via PI3K/AKT/mTOR signaling pathway
    Gao, Yan
    Zhang, Yi
    Fan, Yangyang
    IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 2019, 22 (11) : 1340 - 1346