Injectable Decellularized Extracellular Matrix Hydrogel Containing Stromal Cell-Derived Factor 1 Promotes Transplanted Cardiomyocyte Engraftment and Functional Regeneration after Myocardial Infarction

被引:35
作者
Wu, Kui [1 ,2 ,3 ]
Wang, Yuanyuan [1 ,2 ]
Yang, Hong [1 ,2 ]
Chen, Yihuan [1 ,2 ]
Lu, Kunyan [1 ,2 ]
Wu, Yong [1 ,2 ]
Liu, Chunxia [1 ,2 ]
Zhang, Haixin [4 ]
Meng, Hanyu [1 ,2 ]
Yu, Qian [4 ]
Zhang, Yanxia [1 ,2 ]
Shen, Zhenya [1 ,2 ]
机构
[1] Soochow Univ, Affiliated Hosp 1, Dept Cardiovasc Surg, Suzhou 215007, Peoples R China
[2] Soochow Univ, Suzhou Med Coll, Inst Cardiovasc Sci, Suzhou 215007, Peoples R China
[3] Guizhou Med Univ, Affiliated Hosp, Dept Cardiovasc Surg, Guiyang 550004, Peoples R China
[4] Soochow Univ, Coll Chem Chem Engn & Mat Sci, State & Local Joint Engn Lab Novel Funct Polymer, Suzhou 215123, Peoples R China
基金
中国国家自然科学基金;
关键词
myocardial infarction; decellularized matrix hydrogel; cardiomyocyte; SDF-1; angiogenesis; SDF-1-ALPHA; STRATEGIES; CHEMOKINE; RELEASE; REPAIR;
D O I
10.1021/acsami.2c16682
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Transplantation of exogenous cardiomyocytes (CMs) is a hopeful method to treat myocardial infarction (MI). However, its clinical application still remains challenging due to low retention and survival rates of the transplanted cells. Herein, a stromal cell-derived factor 1 (SDF-1)-loaded injectable hydrogel based on a decellularized porcine extracellular matrix (dECM) is developed to encapsulate and deliver CMs locally to the infarct area of the heart. The soluble porcine cardiac dECM is composed of similar components such as the human cardiac ECM, which could be self-assembled into a nanofibrous hydrogel at physiological temperature to improve the retention of transplanted CMs. Furthermore, the chemokine SDF-1 could recruit endogenous cells to promote angiogenesis, mitigating the ischemic microenvironment and improving the survival of CMs. The results in vitro show that this composite hydrogel exhibits good biocompatibility, anti-apoptosis property, and chemotactic effects for mesenchymal stromal cells and endothelial cells through SDF1-CXCR4 axis. Moreover, intramyocardial injection of this composite hydrogel to the infarcted area leads to the promotion of angiogenesis and inhibition of fibrosis, reducing the infarction size and improving the cardiac function. The combination of natural biomaterials, exogenous cells, and bioactive factors shows potential for MI treatment in the clinical application.
引用
收藏
页码:2578 / 2589
页数:12
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