Dynamic range and reproducibility of hepatitis B virus (HBV) DNA detection and quantification by Cobas Taqman HBV, a real-time semiautomated assay

被引:21
作者
Lindh, M [1 ]
Hannoun, C [1 ]
机构
[1] Gothenburg Univ, Dept Clin Virol, S-41346 Gothenburg, Sweden
关键词
D O I
10.1128/JCM.43.8.4251-4254.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at >= 1,000 IU/ml and 22.0% at 10 IU/ml. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r(2) = 0.97).
引用
收藏
页码:4251 / 4254
页数:4
相关论文
共 24 条
[21]   Rapid quantification of hepatitis B virus DNA by automated sample preparation and real-time PCR [J].
Stelzl, E ;
Muller, Z ;
Marth, E ;
Kessler, HH .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) :2445-2449
[22]   Sensitive and accurate quantitation of hepatitis B virus DNA using a kinetic fluorescence detection system (TaqMan PCR) [J].
Weinberger, KM ;
Wiedenmann, E ;
Böhm, S ;
Jilg, W .
JOURNAL OF VIROLOGICAL METHODS, 2000, 85 (1-2) :75-82
[23]   Real time TaqMan PCR detection and quantitation of HBV genotypes A-G with the use of an internal quantitation standard [J].
Weiss, J ;
Farrenkopf, HWB ;
Schultz, T ;
Song, G ;
Shah, S ;
Siegel, J .
JOURNAL OF CLINICAL VIROLOGY, 2004, 30 (01) :86-93
[24]   Quantitative analysis of hepatitis B virus DNA by real-time amplification [J].
Zanella, I ;
Rossini, A ;
Domenighini, D ;
Albertini, A ;
Cariani, E .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2002, 21 (01) :22-26