Dynamic range and reproducibility of hepatitis B virus (HBV) DNA detection and quantification by Cobas Taqman HBV, a real-time semiautomated assay

被引:21
作者
Lindh, M [1 ]
Hannoun, C [1 ]
机构
[1] Gothenburg Univ, Dept Clin Virol, S-41346 Gothenburg, Sweden
关键词
D O I
10.1128/JCM.43.8.4251-4254.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at >= 1,000 IU/ml and 22.0% at 10 IU/ml. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r(2) = 0.97).
引用
收藏
页码:4251 / 4254
页数:4
相关论文
共 24 条
[1]   Quantitation of hepatitis B virus genomic DNA by real-time detection PCR [J].
Abe, A ;
Inoue, K ;
Tanaka, T ;
Kato, J ;
Kajiyama, N ;
Kawaguchi, R ;
Tanaka, S ;
Yoshiba, M ;
Kohara, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (09) :2899-2903
[2]   Rapid detection and quantitation of hepatitis B virus DNA by real-time PCR using a new fluorescent (FRET) detection system [J].
Aliyu, SH ;
Aliyu, MH ;
Salihu, HM ;
Parmar, S ;
Jalal, H ;
Curran, MD .
JOURNAL OF CLINICAL VIROLOGY, 2004, 30 (02) :191-195
[3]   A rapid real-time quantitative polymerase chain reaction for hepatitis B virus [J].
Brechtbuehl, K ;
Whalley, SA ;
Dusheiko, GM ;
Saunders, NA .
JOURNAL OF VIROLOGICAL METHODS, 2001, 93 (1-2) :105-113
[4]   Real-time PCR for detection and quantitation of hepatitis B virus DNA [J].
Chen, RW ;
Piiparinen, H ;
Seppänen, M ;
Koskela, P ;
Sarna, S ;
Lappalainen, M .
JOURNAL OF MEDICAL VIROLOGY, 2001, 65 (02) :250-256
[5]   Quantitative serum HBV DNA levels during different stages of chronic hepatitis B infection [J].
Chu, CJ ;
Hussain, M ;
Lok, ASF .
HEPATOLOGY, 2002, 36 (06) :1408-1415
[6]   Clinical utility in quantifying serum HBV DNA levels using PCR assays [J].
Chu, CJ ;
Lok, ASF .
JOURNAL OF HEPATOLOGY, 2002, 36 (04) :549-551
[7]   Quantitative assay of PCR-amplified hepatitis B virus DNA using a peroxidase-labelled DNA probe and enhanced chemiluminescence [J].
Erhardt, A ;
Schaefer, S ;
Athanassiou, N ;
Kann, M ;
Gerlich, WF .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (08) :1885-1891
[8]   Rapid quantification of hepatitis B virus DNA by real-time PCR using fluorescent hybridization probes [J].
Ho, SKN ;
Yam, WC ;
Leung, ETK ;
Wong, LP ;
Leung, JKH ;
Lai, KN ;
Chan, TM .
JOURNAL OF MEDICAL MICROBIOLOGY, 2003, 52 (05) :397-402
[9]   The value of quantitative detection of HBV-DNA amplified by PCR is the study of hepatitis B infection [J].
Jardi, R ;
Buti, M ;
RodriguezFrias, F ;
Cortina, M ;
Esteban, R ;
Guardia, J ;
Pascual, C .
JOURNAL OF HEPATOLOGY, 1996, 24 (06) :680-685
[10]   Quantitative detection of hepatitis B virus DNA in serum by a new rapid real-time fluorescence PCR assay [J].
Jardi, R ;
Rodriguez, F ;
Buti, M ;
Costa, X ;
Cotrina, M ;
Valdes, A ;
Galimany, R ;
Esteban, R .
JOURNAL OF VIRAL HEPATITIS, 2001, 8 (06) :465-471