Homogeneous protein assays mediated by dynamic DNA nanotechnology

被引：0

作者：

Gao, Jiajie ^{[1
]}

Gao, Lu ^{[1
]}

Tang, Yanan ^{[1
]}

Li, Feng ^{[1
,2
]}

机构：

[1] Sichuan Univ, Key Lab Green Chem & Technol, Minist Educ, Coll Chem,Analyt & Testing Ctr, Chengdu 610064, Sichuan, Peoples R China

[2] Brock Univ, Ctr Biotechnol, Dept Chem, 1812 Sir Isaac Brock Way, St Catharines, ON L2S 3A1, Canada

来源：

CANADIAN JOURNAL OF CHEMISTRY | 2022年 / 100卷 / 12期

基金：

中国国家自然科学基金;

关键词：

homogeneous protein assay; DNA nanotechnology; DNA nanomachine; protein detection; DNA strand displace-ment; PROXIMITY LIGATION ASSAYS; AMPLIFIED DETECTION; ANTIBODIES; NANOMACHINE; ASSEMBLIES; BIOSENSOR; SENSORS; BEACON;

D O I：

10.1139/cjc-2022-0150

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Driven by recent advances in DNA nanotechnology, analytical methods have been greatly improved for designing simple and homogeneous assays for proteins. The translation from target proteins to DNA outputs dramatically enhances the sensitivity of protein assays. More importantly, the protein-responsive DNA nanotechnology has offered diverse assay mechanisms, allowing flexible assay designs and high sensitivity without the need for sophisticated operational procedures. This review will focus on the design principles and mechanistic insight of analytical assays mediated by protein-responsive DNA nanotechnology, which will serve a general guide for assay design and applications.

引用

页码：900 / 909

页数：10

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