Photoencapsulation of bone morphogenetic protein-2 and periosteal progenitor cells improve tendon graft healing in a bone tunnel

被引:59
作者
Chen, Chih-Hwa [2 ]
Liu, Hsia-Wei [1 ,2 ]
Tsai, Ching-Lin [3 ]
Yu, Chung-Ming [2 ]
Lin, I-Hsuan [2 ]
Hsiue, Ging-Ho [1 ]
机构
[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 30013, Taiwan
[2] Chang Gung Univ, Chang Gung Mem Hosp Keelung, Dept Orthopaed Surg, Coll Med, Chilung, Taiwan
[3] Natl Taiwan Univ Hosp, Dept Orthopaed Surg, Coll Med, Taipei, Taiwan
关键词
bone morphogenetic protein; hydrogel; periosteal progenitor cell; photoencapsulation; tendon-bone healing;
D O I
10.1177/0363546507311098
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Background: Tissue-engineered solutions for promoting the tendon graft incorporation within the bone tunnel appear to be promising. Hypothesis: To determine the feasibility that conjugation of hyaluronic acid-tethered bone morphogenetic protein-2 can be used to stimulate periosteal progenitor cells direct fibrocartilagenous attachment and new bone formation in an extra-articular tendon-bone healing model. Study Design: Controlled laboratory study. Methods: A total of 42 mature New Zealand White rabbits were used. The long digitorum extensor tendon was transplanted into a bone tunnel of the proximal tibia. The tendon was pulled through a drill hole in the proximal tibia and attached to the medial aspect of the tibia. Photopolymerizable hydrogel based on poly (ethylene glycol) diacrylate with hyaluronic acid-tethered bone morphogenetic protein-2 was injected and photogelated in a bone tunnel. Histological and biomechanical examination of the tendon-bone interface was evaluated at postoperative weeks 3 and 6. Results: Histological analysis showed an interface fibrocartilage and new bone formed by photoencapsulation of bone morphogenetic protein-2 and periosteal progenitor cells at 6 weeks. Biomechanical testing revealed higher maximum pullout strength and stiffness in experimental groups with a statistically significant difference at 3 and 6 weeks after tendon transplantation. Conclusion: The healing tendon-bone interface undergoes a gradual remodeling process; it appears that photoencapsulation of bone morphogenetic protein-2 and periosteal progenitor cells possesses a powerful inductive ability between the tendon and the bone to incorporate the healing in a rabbit model. Clinical Relevance: Novel technologies, such as those described in this study, including photopolymerization and tissue engineering, may provide minimally invasive therapeutic procedures via arthroscopy to enhance biological healing after reconstruction of the anterior cruciate ligament.
引用
收藏
页码:461 / 473
页数:13
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