Hyperforin Inhibits Cell Growth by Inducing Intrinsic and Extrinsic Apoptotic Pathways in Hepatocellular Carcinoma Cells

被引：57

作者：

Chiang, I-Tsang ^{[1
,4
,5
]}

Chen, Wei-Ting ^{[6
]}

Tseng, Chih-Wei ^{[7
]}

Chen, Yen-Chung ^{[2
,4
]}

Kuo, Yu-Cheng ^{[8
]}

Chen, Bi-Jhih ^{[9
]}

Weng, Mao-Chi ^{[10
]}

Lin, Hwai-Jeng ^{[11
,12
]}

Wang, Wei-Shu ^{[3
,4
,13
]}

机构：

[1] Natl Yang Ming Univ Hosp, Dept Radiat Oncol, Yilan, Taiwan

[2] Natl Yang Ming Univ Hosp, Dept Pathol, Yilan, Taiwan

[3] Natl Yang Ming Univ Hosp, Dept Med, 152 Xinmin Rd, Yilan 260, Yilan County, Taiwan

[4] Natl Yang Ming Univ Hosp, Canc Med Care Ctr, Yilan, Taiwan

[5] Cent Taiwan Univ Sci & Technol, Dept Radiol Technol, Taichung, Taiwan

[6] Kaohsiung Armed Forces Gen Hosp, Zuoying Branch, Dept Psychiat, Kaohsiung, Taiwan

[7] Dalin Tzu Chi Hosp, Buddhist Tzu Chi Med Fdn, Dept Internal Med, Div Gastroenterol, Chiayi, Taiwan

[8] Show Chwan Mem Hosp, Radiat Oncol, Changhua, Taiwan

[9] Changhua Christian Hosp, Changhua Christian Med Fdn, Dept Lab Med, Changhua, Taiwan

[10] Atom Energy Council, Inst Nucl Energy Res, Taoyuan, Taiwan

[11] Taipei Med Univ, Div Gastroenterol & Hepatol, Dept Internal Med, Coll Med,Sch Med, Taipei, Taiwan

[12] Shuang Ho Hosp, Div Gastroenterol & Hepatol, Dept Internal Med, 901,291 Zhongzheng Rd, New Taipei 23561, Taiwan

[13] Natl Yang Ming Univ, Sch Med, Taipei, Taiwan

来源：

ANTICANCER RESEARCH | 2017年 / 37卷 / 01期

关键词：

Hepatocellular carcinoma; hyperforin; apoptosis; CYTOCHROME-C RELEASE; CYCLE ARREST; CANCER; ACTIVATION; ANTIDEPRESSANT; MITOCHONDRIA; FLUOXETINE; INDUCTION; INVASION; DNA;

D O I：

10.21873/anticanres.11301

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G(1) phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells.

引用

页码：161 / 167

页数：7

共 29 条

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