FMRFamide-related peptides in potato cyst nematodes

被引:39
作者
Kimber, MJ
Fleming, CC
Bjourson, AJ
Halton, DW
Maule, AG
机构
[1] Queens Univ Belfast, Ctr Med Biol, Sch Biol & Biochem, Parasitol Res Grp, Belfast BT9 7BL, Antrim, North Ireland
[2] Queens Univ Belfast, Belfast BT9 7BL, Antrim, North Ireland
[3] Dept Agr & Rural Dev, Belfast BT9 5PX, Antrim, North Ireland
关键词
nematode; Globodera pallida; Globodera rostochiensis; neuropeptide; FMR-Famide-related peptide;
D O I
10.1016/S0166-6851(01)00323-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study presents data demonstrating the presence of FMRFamide-related peptides (FaRPs) in potato cyst nematodes (PCN). Five transcripts of FaRP encoding genes, designated gpflp-1 to gpflp-5, were characterised using RACE. In terms of ORFs, gpflp-1 was 444 base pairs (bp) long and coded for four copies of the FaRP, PF3 (KSAYMRFamide) whilst gpflp-2 was 309 bp long and encoded one copy of the peptide, KNKFEFIRFamide. gpflp-3 (420 bp) Encoded two copies of KHEYLRFamide (AF2) and the genes gpflp-4 and gpflp-5 encoded a total of 11 FaRPs, most of which are novel to PCN. FMRFamide-related peptide (FaRP)-like immunoreactivity was observed in both PCN species, Globodera pallida and Globodera rostochiensis, using an antiserum raised against the invertebrate peptide, FMRFamide. Immunopositive neurones were found throughout the central nervous system in the ventral and dorsal nerve cords and the circumpharyngeal. and perianal nerve rings. Reactive neurones were also present peripherally, innervating the highly muscular pharynx with a nerve net and ring-like structures. Positive immunostaining was also observed in neurones running toward the stylet protractor muscles and/or the anterior sensory apparatus. This study implicates a role for FaRPs in feeding, host penetration and sensory function of PCN. This is the first study to characterise FaRP encoding genes from a plant-parasitic nematode using a targeted PCR based RACE approach and further underlines the importance and diversity of this neuropeptide group in the phylum Nematoda. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:199 / 208
页数:10
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