SERS-Based Lateral Flow Strip Biosensor for Simultaneous Detection of Listeria monocytogenes and Salmonella enterica Serotype Enteritidis

被引：157
作者：
Liu, Hai-bin [1 ]
Du, Xin-jun [1 ]
Zang, Yu-Xuan [1 ]
Li, Ping [1 ]
Wang, Shuo [1 ,2 ]
机构：
[1] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ, Tianjin 300457, Peoples R China
[2] BTBU, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2017年 / 65卷 / 47期
关键词：
recombinase polymerase amplification; foodborne pathogen; surface-enhanced Raman scattering; food safety; POLYMERASE AMPLIFICATION ASSAY; ESCHERICHIA-COLI O157H7; SENSITIVE DETECTION; IMMUNOASSAY STRIP; IDENTIFICATION; NANOPARTICLES; TYPHIMURIUM; PLATFORM; PROBE;
D O I：
10.1021/acs.jafc.7b03957
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Rapid, sensitive, point-of-care detection of bacteria is extremely important in food safety. To address this requirement, we developed a new surface-enhanced Raman scattering (SERS)-based lateral flow (LF) strip biosensor combined with recombinase polymerase amplification (RPA) for simultaneous detection of Listeria monocytogenes and Salmonella enterica serotype Enteritidis. Au-MBA@Ag core shell nanop articles were used in this SERS-LF. Highly sensitive quantitative detection is achieved by measuring the characteristic peak intensities of SERS tags. Under optimal conditions, the SERS intensities of MBA at 1077 cm(-1) on test lines are used to measure S. Enteritidis (y = 1980.6x - 539.3, R-2 = 0.9834) and L. monocytogenes (y = 1696.0x 844, R-2 = 0.9889), respectively. The limit of detection is 27 CFU/mL for S. Enteritidis and 19 CFU/mL for L. monocytogenes. Significantly, this SERS-LF has high specificity and applicability in the detection of L. monocytogenes and S. Enteritidis in food samples. Therefore, the SERS-LF is a feasible method for the rapid and quantitative detection of a broad range of bacterial pathogens in real food samples.
引用
收藏
页码：10290 / 10299
页数：10
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