Video-rate spectral imaging of fluorescence phenomena

被引：3

作者：

Ford, BK ^{[1
]}

Volin, CE ^{[1
]}

Descour, MR ^{[1
]}

Garcia, JP ^{[1
]}

Wilson, DW ^{[1
]}

Maker, PD ^{[1
]}

Bearman, GH ^{[1
]}

机构：

[1] Univ Arizona, Ctr Opt Sci, Tucson, AZ 85721 USA

来源：

IMAGING SPECTROMETRY IV | 1998年 / 3438卷

关键词：

spectral imaging; high-speed imaging; fluorescence spectroscopy; imaging spectrometry;

D O I：

10.1117/12.328119

中图分类号：

TP7 [遥感技术];

学科分类号：

081102 ; 0816 ; 081602 ; 083002 ; 1404 ;

摘要：

We describe fluorescence spectral-imaging results with the microscope computed-tomography imaging spectrometer (mu CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, the mu CTIS can be used to image dynamic phenomena involving multiple, spectrally overlapping fluorescence probes. The results presented in this paper consist of proof-of-concept imaging results using two static targets. The first is composed of 6-mu m fluorescing microspheres and the second consists of rat sinusoid epithelial (RSE-1) cells loaded with 0.5-mu m fluorescing microspheres. Image data were collected in integration times of 16 msec, comparable to video frame rate integration times. The emission spectra were sampled at 10-nm intervals between 420 nm and 710 nm. The smallest spatial sampling interval presented in this paper is 1.7 mu m.

引用

页码：313 / 320

页数：8