Analysis of collagen distribution in human crown dentin by confocal laser scanning microscopy

被引：26
作者：
Lucchese, Alberta [1 ]
Pilolli, Giovanni Pietro [1 ]
Petruzzi, Massimo [1 ]
Crincoli, Vito [1 ]
Scivetti, Michele [1 ]
Favia, Gianfranco [1 ]
机构：
[1] Univ Bari, Dept Odontostomatol & Surg, I-70124 Bari, Italy
来源：
ULTRASTRUCTURAL PATHOLOGY | 2008年 / 32卷 / 03期
关键词：
autofluorescence; collagen; confocal laser scanning microscope; dentin; microhardness;
D O I：
10.1080/01913120801897216
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
The authors used confocal laser scanning microscope to analyze human crown dentin. Specimens from 10 teeth were divided in two groups, one of which was decalcified and stained with hematoxylin and eosin. In the second group an undecalcified section was analyzed. Both groups were scanned by confocal microscope to generate optically sectioned images. All of the analyzed samples presented an intense autofluorescent that was ascribed to collagens. The degree of autofluorescence intensity was variable and might be due to collagen expression. The results indicate that a confocal microscope may be of help in analyzing and defining the nature and extent of collagen fibrils in human dentin.
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收藏
页码：107 / 111
页数：5
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