Reverse transcriptase activity and particle production in B lymphoblastoid cell lines established from lymphocytes of patients with multiple sclerosis

被引：22
作者：
Christensen, T
Tönjes, RR
Megede, JZ
Boller, K
Moller-Larsen, A
机构：
[1] Aarhus Univ, Dept Med Microbiol, DK-8000 Aarhus C, Denmark
[2] Paul Ehrlich Inst, D-63225 Langen, Germany
来源：
AIDS RESEARCH AND HUMAN RETROVIRUSES | 1999年 / 15卷 / 03期
关键词：
D O I：
10.1089/088922299311466
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We have established spontaneously formed B lymphoblastoid cell lines from long-term cultured peripheral blood mononuclear cells (PBMNCs) from multiple sclerosis (MS) patients. The MS cell lines actively produce retrovirus-like particles as well as Epstein-Barr virus (EBV). Using three different variations of the highly sensitive polymerase chain reaction (PCR)-based assays for the detection of reverse transcriptase (RT) activity, we have verified the retroviral origin of the retrovirus-like particles that are produced in very low amounts by the MS cell lines.
引用
收藏
页码：285 / 291
页数：7
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