Analysis of Lipophilic and Hydrophilic Bioactive Compounds Content in Sea Buckthorn (Hippophae rhamnoides L.) Berries

被引：130

作者：

Teleszko, Miroslawa ^{[1
]}

Wojdylo, Aneta ^{[1
]}

Rudzinska, Magdalena ^{[2
]}

Oszmianski, Jan ^{[1
]}

Golis, Tomasz ^{[3
]}

机构：

[1] Wroclaw Univ Environm & Life Sci, Dept Fruit & Vegetable Technol, Chelmonskiego 37 St, PL-51630 Wroclaw, Poland

[2] Poznan Univ Life Sci, Inst Food Technol Plant Origin, PL-60624 Poznan, Poland

[3] Inst Hort Res, Dept Pomol Gene Resources & Nurseries, PL-96100 Skierniewice, Poland

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2015年 / 63卷 / 16期

关键词：

sea buckthorn; bioactive compounds; fatty acids; phytosterols; polypheriols; FLAVONOL GLYCOSIDES; PHENOLIC-ACIDS; ANTIOXIDANT CAPACITY; BLACK-CURRANT; SSP SINENSIS; FAT SPREAD; VITAMIN-C; CULTIVARS; HPLC; PHYTOSTEROLS;

D O I：

10.1021/acs.jafc.5b00564

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The aim of this study was to determine selected phytochemicals in berries of eight sea buckthorn (Hippophae rhamnoides subsp. mongolica) cultivars, including lipophilic and hydrophilic compounds. In the experiment chromatographic analyses, GC (phytosterols and fatty acids); UPLC-PDA-FL, LC-MS (polyphenols), and HPLC (L-ascorbic acid), as well spectrophotometric Method (total carotenoids) were used. The lipid fraction isolated from whole fruit contained 14 phytosterols (major compounds beta-sitosterol > 24-methylenecykloartanol > squalene) and 11 fatty acids in the order MUFAs > SFAs > PUFAs. Carotenoids occurred in concentrations between 6,19 and 23.91 mg/100 g fresh weight (fw) (p < 0.05). The major' polyphenol group identified in berries was flavonols (mean content of 311.55 mg/100 g fw); with the structures of isorhamnetin (six compounds), quercetin (four compounds), and kaempferol (one compound) glycosides. Examined sea buckthorn cultivars were characterized also by a high content of L-ascorbic acid in a range from 52:86 to 130.97 mg/100 g fw (p < 0.05).

引用

页码：4120 / 4129

页数：10

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