Phage display for target-based antibacterial drug discovery

被引：44

作者：

Christensen, DJ ^{[1
]}

Gottlin, EB ^{[1
]}

Benson, RE ^{[1
]}

Hamilton, PT ^{[1
]}

机构：

[1] Karo Bio USA, Durham, NC 27703 USA

来源：

DRUG DISCOVERY TODAY | 2001年 / 6卷 / 14期

关键词：

D O I：

10.1016/S1359-6446(01)01853-0

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Increasing bacterial drug resistance and hard-to-eradicate opportunistic infections have created a need for new antibiotics. Sequencing of microbial genomes has yielded many new potential targets for antibacterial drug discovery. However, little is known about the biochemical activities of many of these targets, making it difficult to develop HTS assays for them. Peptides isolated by phage display can be used as 'surrogate ligands' in competition assays for screening of targets of unknown function with small-molecule libraries. These screening assays can be adapted into a variety of high-throughput formats, including those based on radioactive, luminescence or fluorescence detection.

引用

页码：721 / 727

页数：7

共 80 条

[1] High throughput fluorescence polarization: A homogeneous alternative to radioligand binding for cell surface receptors [J].