Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure

被引：24

作者：

Leung, Priscilla T. Y. ^{[1
,2
,3
]}

Yi, Andy Xianliang ^{[1
,2
]}

Ip, Jack C. H. ^{[1
,2
]}

Mak, Sarah S. T. ^{[1
,2
,4
]}

Leung, Kenneth M. Y. ^{[1
,2
,3
]}

机构：

[1] Univ Hong Kong, Swire Inst Marine Sci, Pokfulam, Hong Kong, Peoples R China

[2] Univ Hong Kong, Sch Biol Sci, Pokfulam, Hong Kong, Peoples R China

[3] City Univ Hong Kong, State Key Lab Marine Pollut, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China

[4] Univ Copenhagen, Ctr GeoGenet, Nat Hist Museum Denmark, Copenhagen, Denmark

来源：

MARINE POLLUTION BULLETIN | 2017年 / 124卷 / 02期

关键词：

Copper; Marine diatom; Thermal stress; Thalassiosira; Photosynthesis; Heat shock protein; ENDOCRINE-DISRUPTING CHEMICALS; DEPENDENT TOXICITIES; SILICON TRANSPORTERS; ZINC-OXIDE; MICROALGAE; WATER; COPEPOD; METALS; FLUORESCENCE; SENSITIVITY;

D O I：

10.1016/j.marpolbul.2017.03.038

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (sea-water control; 200 mu g/L Cu, EC50 for the yield at 25 degrees C; and 1000 mu g/L Cu, EC50 for growth inhibition at 25 degrees C) were conducted against four temperatures (10 degrees C, 15 degrees C, 25 degrees C and 30 degrees C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30 degrees C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90-2 and sit1 (related to silica shell formation) were highly expressed at 30 degrees C under 1000 mu g/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15 degrees C under 200 mu g/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：938 / 945

页数：8

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