LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli

被引：84
|
作者：
Altincicek, B
Kollas, AK
Eberl, M
Wiesner, J
Sanderbrand, S
Hintz, M
Beck, E
Jomma, H
机构：
[1] Jomaa Pharmaka GmbH, D-35392 Giessen, Germany
[2] Univ Giessen, Inst Biochem, D-35392 Giessen, Germany
来源：
FEBS LETTERS | 2001年 / 499卷 / 1-2期
关键词：
isoplenoid biosynthesis; 2-C-methyl-D-erythritol 4-phosphate pathway; lytB;
D O I：
10.1016/S0014-5793(01)02516-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mevalonate-independent 2-C-mcthyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis is essential in many eubacteria, plants, and the malaria parasite. Using genetically engineered Escherichia coli cells able to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway. we demonstrate that the lytB gene is involved in the trunk line of the MEP pathway. Cells deleted for the essential lytB gene were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of lytB. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：37 / 40
页数：4
相关论文
共 50 条
  • [1] Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli
    Campos, N
    Rodríguez-Concepción, M
    Seemann, M
    Rohmer, M
    Boronat, A
    FEBS LETTERS, 2001, 488 (03) : 170 - 173
  • [2] GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli
    Altincicek, B
    Kollas, AK
    Sanderbrand, S
    Wiesner, J
    Hintz, M
    Beck, E
    Jomaa, H
    JOURNAL OF BACTERIOLOGY, 2001, 183 (08) : 2411 - 2416
  • [3] fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis
    Puan, KJ
    Wang, H
    Dairi, T
    Kuzuyama, T
    Morita, CT
    FEBS LETTERS, 2005, 579 (17) : 3802 - 3806
  • [4] Deuterium-labelled isotopomers of 2-C-methyl-D-erythritol as tools for the elucidation of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis
    Charon, L
    Hoeffler, JF
    Pale-Grosdemange, C
    Lois, LM
    Campos, N
    Boronat, A
    Rohmer, M
    BIOCHEMICAL JOURNAL, 2000, 346 : 737 - 742
  • [5] Cutting edge:: Human γδ T cells are activated by intermediates of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis
    Altincicek, B
    Moll, J
    Campos, N
    Foerster, G
    Beck, E
    Hoeffler, JF
    Grosdemange-Billiard, C
    Rodríguez-Concepción, M
    Rohmer, M
    Boronat, A
    Eberl, M
    Jomaa, H
    JOURNAL OF IMMUNOLOGY, 2001, 166 (06): : 3655 - 3658
  • [6] Mechanistic studies with 2-C-methyl-D-erythritol 4-phosphate synthase from Escherichia coli
    Fox, DT
    Poulter, CD
    BIOCHEMISTRY, 2005, 44 (23) : 8360 - 8368
  • [7] Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate:: a novel system for the genetic analysis of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis
    Campos, N
    Rodríguez-Concepción, M
    Sauret-Güeto, S
    Gallego, F
    Lois, LM
    Boronat, A
    BIOCHEMICAL JOURNAL, 2001, 353 : 59 - 67
  • [8] Formation of 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol 4-phosphate by 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate pathway
    Kuzuyama, T
    Takagi, M
    Kaneda, K
    Dairi, T
    Seto, H
    TETRAHEDRON LETTERS, 2000, 41 (05) : 703 - 706
  • [9] On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis
    Charon, L
    Pale-Grosdemange, C
    Rohmer, M
    TETRAHEDRON LETTERS, 1999, 40 (40) : 7231 - 7234
  • [10] Chemical synthesis of enantiopure 2-C-methyl-D-erythritol 4-phosphate, the key intermediate in the mevalonate-independent pathway for isoprenoid biosynthesis
    Hoeffler, JF
    Pale-Grosdemange, C
    Rohmer, M
    TETRAHEDRON, 2000, 56 (11) : 1485 - 1489
12345