Purification, Modeling, and Analysis of Botulinum Neurotoxin Subtype A5 (BoNT/A5) from Clostridium botulinum Strain A661222

被引：29

作者：

Jacobson, Mark J. ^{[1
]}

Lin, Guangyun ^{[1
]}

Tepp, William ^{[1
]}

Dupuy, Jerome ^{[2
]}

Stenmark, Pal ^{[2
,3
]}

Stevens, Raymond C. ^{[2
]}

Johnson, Eric A. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

[2] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[3] Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 12期

基金：

瑞典研究理事会;

关键词：

ANTIBODY-BINDING; TOXIN; EVOLUTION; REGIONS; GENES; CHAIN;

D O I：

10.1128/AEM.00201-11

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Clostridium botulinum type A strain (A661222) in our culture collection was found to produce the botulinum neurotoxin subtype A5 (BoNT/A5). Its neurotoxin gene was sequenced to determine its degree of similarity to available sequences of BoNT/A5 and the well-studied BoNT/A1. Thirty-six amino acid differences were observed between BoNT/A5 and BoNT/A1, with the predominant number being located in the heavy chain. The amino acid chain of the BoNT/A from the A661222 strain was superimposed over the crystal structure of the known structure of BoNT/A1 to assess the potential significance of these differences-specifically how they would affect antibody neutralization. The BoNT/A5 neurotoxin was purified to homogeneity and evaluated for certain properties, including specific toxicity and antibody neutralization. This study reports the first purification of BoNTA5 and describes distinct differences in properties between BoNT/A5 and BoNT/A1.

引用

页码：4217 / 4222

页数：6