Mortality Caused by Bath Exposure of Zebrafish (Danio rerio) Larvae to Nervous Necrosis Virus Is Limited to the Fourth Day Postfertilization

被引：10

作者：

Morick, Danny ^{[1
,2
]}

Faigenbaum, Or ^{[1
,2
]}

Smirnov, Margarita ^{[3
]}

Fellig, Yakov ^{[1
,2
]}

Inbal, Adi ^{[4
]}

Kotler, Moshe ^{[1
,2
]}

机构：

[1] Hebrew Univ Jerusalem, Sch Med, Dept Pathol, IL-91010 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Sch Med, Lautenberg Ctr Gen & Tumor Immunol, IL-91010 Jerusalem, Israel

[3] Minist Agr & Rural Dev, Dept Fisheries & Aquaculture, Nir David, Israel

[4] Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Med Neurobiol, Jerusalem, Israel

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2015年 / 81卷 / 10期

关键词：

MATERNAL TRANSFER; PROTECTIVE ROLE; FISH; DISEASE; EGG; LOCALIZATION; TEMPERATURE; INFECTION; LYMNAEA; GROUPER;

D O I：

10.1128/AEM.04175-14

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV by bath immersion at 2, 4, 6, and 8 days postfertilization (dpf). Here, we demonstrate that developing zebrafish embryos are resistant to NNV at 2 dpf due to the protection afforded by the egg chorion and, to a lesser extent, by the perivitelline fluid. The zebrafish larvae succumbed to NNV infection during a narrow time window around the 4th dpf, while 6- and 8-day-old larvae were much less sensitive, with mortalities of 24% and 28%, respectively.

引用

页码：3280 / 3287

页数：8